专利名称：Shortened purification process for the production of capsular Streptococcus
流星的英文pneumoniae polysaccharides
发明人：Yonghui Yuan,Mark Ruppen,Wei-Qiang Sun,Ling Chu,John Simpson,James
Patch,Justin Keith Moran,Pamela Fink
申请号：US12052525
申请日：20080320
公开号：US08652480B2
公开日：
冷风扇
20140218
专利内容由知识产权出版社提供
太阳系的英文个人工作业绩简述摘要：A shortened process for producing a solution containing substantially purified capsular polysaccharides from a cellular lysate broth is described. Ultrafiltering and diafiltering a clarified lysate followed by pH adjustment to less than 4.5, preferably about 3.5, precipitated at least 98% of the protein in the solution without riously affecting polysaccharide yield. Furthermore, following ultrafiltration and diafiltration and acidification to a pH of less than 4.5, filtration using activated carbon precipitated at least 90% of remaining protein without riously affecting polysaccharide yield. Exemplary, non-limiting rotypes that can be purified using the shortened process of the invention are 1, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F. In one embodiment, the cells are lyd using deoxycholate sodium (DOC), while in another embodiment the lytic agent is a non-animal derived lytic agent such as N-lauryl sarcosine sodium (NLS).
三个强盗申请人：Yonghui Yuan,Mark Ruppen,Wei-Qiang Sun,Ling Chu,John Simpson,James Patch,Justin Keith Moran,Pamela Fink妈祖简介
地址：Tappan NY US,Garnerville NY US,Morristown NJ US,Suffern NY US,Upper Nyack NY US,Cornwall on Hudson NY US,Valley Cottage NY US,New City NY US 国籍：US,US,US,US,US,US,US,US
代理人：Royal N. Ronning, Jr.
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