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北京博莱明创生物技术有限公司
MininGene Biotechnology Co. Ltd
Phone: 86-10-86982722 Email:
RE: Report for xxx genome analysis of 454 data.
对个人的表扬信>特种服务员Dear Dr ,
A draft genomic quences were generated from Roche/454 GS-FLX system with Titanium chemistry (fragment quencing) accompanied with Illumina/Solexa Genome Analyzer (mate pair quencing). The ABI 3730xl was employed to to do the genome finishing by primer walking. The following plea e the statistics of quencing and analysis results.
Draft Genome Map & Scaffolding
Tab1. 454 Sequencing Statistics
Strain CLH # of 454 Reads 217,210 Total Length 84,464,882 Mean Length
388.86
Median Length
423
Tab2. 454 Asmbly Statistics茼蒿怎么做
Strain
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# of Contigsa
Total Length
Mean Length
Median Length
N50 Lengthb
Max Length
CLH 200573 101 2759203 26578 7831 67161 Asmbled by Newbler with defaut parameter b N50 is calculated by first ordering all contig (or scaffold) sizes and then adding the lengths (starting from the longest contig) until the summed length exceeds 50% of the total length of all contigs
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The Lander-Waterman curve indicates that the data of quences is enough for the CLH Asmbly
Tab3. Scaffolding by 454 reads and Solexa paired-ends reads (2 libraries, 300bp and 5kb inrt) Mean Median N50 # of Scaffold Total Length Max Length Strain Length Length Length CLH 1015651 22 2715500 123431 67592 211855
Finished Genome
1. Genome Characteriscs
Tab4. The genome feature of CLH
Featurea Length of quence (bp) G+C content Repeat content length (%) Simple repeats length (coverage) Low complexity length (coverage) Repeat element length (coverage) Small RNA amount (length, coverage) a Annotated by Repeatmasker. Strains CLH 2,842,906 29.44 % 2.11 % 2,024 (0.07%) 38,860 (1.37%) 1,933 (0.07%) 52 (17336,0.61%)
Fig : Circle genome view of the finish map..
Fig : genome compare
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2. Genome annotation
Feature ORF (Length in Genome) Feature ORFa # 2887 Genome coverage (%) 85.02% Genome density (genes/Kb) 1.02 Average gene length (bp) 837 Max gene length (bp) 8616 Function ORF with assigned functionsb 2001 2496 Conrved hypothetical proteinsc ORF without databa match
390 RNA rRNAd 5S rRNA # (length) 9 (115) 16S rRNA # (length) 10 (1502) 23S rRNA # (length) 10 (2890) 52 (4057) tRNAe # (total length) a Predicted by Glimmer3 b BLAST against COG databa with e-value less than 1e-10 c BLAST against Nr databa with e-value less than 1e-5 d Predicted by tRNAscan-SE e Predicted by RNAmmer Strains CLH 2,416,902
怎么查看显卡配置3. Gene Annotation
CLH Databa # of Annotated Total Genes Nr Swissprot Kegg COG Interpro GO 2887 2496 1512 2316 2001 2077 1600 86.5 52.4 80.2 69.3 71.9 Interproscan 4.3 55.4 Relea16.0 1e-5 1e-10 1e-10 1e-10 20091003 20091208 Relea50 No Version % E cutoff Databa Version
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民事起诉状怎么写
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1)
COG (Clusters of Orthologous Groups of proteins)
怎么生发和增发
Fig : COG cluster of CLH
Tab5. COG category of CLH
Class Definition (I) Class Definition (II) Cell cycle control, cell division, chromosome partitioning Cell motility Cell wall/membrane/envelope biogenesis Defen mechanisms Intracellular trafficking, cretion, and vesicular transport Posttranslational modification, protein turnover, chaperones Signal transduction mechanisms Replication, recombination and repair Transcription Translation, ribosomal structure and biogenesis Amino acid transport and metabolism Carbohydrate transport and metabolism Coenzyme transport and metabolism Energy production and conversion Inorganic ion transport and metabolism Lipid transport and metabolism Nucleotide transport and metabolism Secondary metabolites biosynthesis, transport and catabolism Function unknown General function prediction only Number of Genes 26 22 111 83 13 74 64 142 110 152 152 78 83 95 90 47 65 22 169 203
Cellular Process and Signaling
Information Storage and Processing
Metabolism
Poorly Characterized
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2) GO(Gene Ontology)
3) Kegg Pathway
Fig: GO Classification of the isolate.
4. Evolution
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