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RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53
晚宴邀请短信mutant mou model.
期刊名称: Journal of Proteome Rearch
作者: Hu, Chunxiu,van Dommelen, Judith,van der Heijden, Rob,Spijksma,
Gerwin,Reijmers, Theo H.,Wang, Mei,Slee, Elizabeth,Lu, Xin,Xu, Guowang,van der Greef, Jan,Hankemeier, Thomas
自制火锅底料家常做法年份: 2008年
期号: 第11期
关键词: Lipidomics; Lipid Profiling; Metabolite Profiling; Metabolomics; FTMS;
p53 Mou Model
丈母娘过生日
摘要:A reverd-pha liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mou plasma. With the u of a fud-core C 8 column and a binary gradient, more than 160 lipids belonging to eight different class were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity ( R (2), 0.994-1.000), limit of detection (0.08-1.28 microg/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory. The method was successfully applied
to p53 mutant mice plasma for studying some phenotypic effects of p53 expression.秦琼观阵
内容由中国教育图书进出口有限公司引进防尘