qfii机构
在磨练中成长Hybrid genes in the analysis of transformation
conditions: II. Transient expression vs stable transformation—analysis of parameters influencing gene expression levels and transformation
efficiency
期刊名称： Physiologia Plantarum
我喜欢的小动物年份： 2010年
关键词： DNA;electric field;Nicotiana plumbaginifolia;Nicotiana
镇远古镇在贵州哪个市
精神的英文tabacum;polyethylene glycol;protoplasts宽英文
摘要：Reports on direct gene transfer have dealt with either the obtention of stable transformants and transgenic plants, or described the u of reporter genes
to analy different aspects of gene expression in plant protoplasts and conditions for their u in trans
ient gene expression assays. In this paper we prent comparisons between veral transformation techniques, show species-specific differences in efficiencies of stable transformants and in the levels of transient gene expression, and report on the identification of major parameters responsible for DNA uptake as judged from transient chloramphenicol acetyl transfera (CAT) expression levels and from efficiencies of transformation bad on kanamycin-resistance. The described procedures have been simplified, optimized and standardized and should allow routine u with
内容由中国教育图书进出口有限公司引进悟空电影天堂
高山仰止的意思
