专利名称：Recombinant human granulocyte-
macrophage-colony stimulating factor (GM-
CSF)
发明人：Steven C. Clark,Randal J. Kaufman,Gordon G.
Wong,Elizabeth A. Wang
申请号：US08/466308
补充的近义词申请日：19950606
公开号：US05891429A
公开日：
19990406
兔子游泳
专利内容由知识产权出版社提供
摘要：A method for preparing and isolating a transformation vector containing
CSF/cDNA is described. The method compris:
南辕北辙翻译
preparing RNA from a cell that produces CSF;
preparing polyadenylated mesnger RNA from said RNA;
电脑扬声器显示未插上preparing single stranded cDNA from said mesnger RNA;
converting the single stranded cDNA to double stranded cDNA;
inrting the double stranded cDNA into transformation vectors and transforming bacteria with said vector to form colonies;cad插入表格
picking pools of 200 to 500 colonies each and isolating plasmid DNA from each pool;
历历可见transfecting the plasmid DNA into suitable host cells for expressing CSF protein;
culturing the transfected cells and assaying the supernatant for CSF activity; and< P>
lecting CSF positive pools and screening the colonies ud to make the pool to identify a colony having CSF activity. Also described are a cDNA coding for a protein having CSF activity (i.e. CSF/cDNA), a microorganism or cell line transformed with a recombinant vector containing such CSF/cDNA, and a method for producing CSF protein by expressing said CSF/cDNA by culturing a microorganism or cell line. The invention also provides a method of purifying the CSF proteins and the purified proteins so produced.
申请人：THE NOVARTIS CORPORATION
代理机构：Fitzpatrick, Cella, Harper & Scinto
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