专利名称：Trace sample derived from cDNA
amplification method
发明人：角田 弘之,フアン フアン，太田 真理，神原 秀記
申请号：JP2014507318
申请日：20121121
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公开号：JPWO2013145431A1
公开日：
星油藤
手机壁纸女
20151210
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专利附图：
摘要： The prent invention aims to provide a sample preparation method for
comprehensively and accurately analyze the gene expression of one or a small number of cells in large-scale DNA quencer. prent invention provides a method of amplifying cDNA from mRNA in cells (1) in one reaction vesl, first tag the mRNA from the cell,immobilized on a solid support captured by the first DNA probe consisting of quences
紫薯怎么保存and poly-T quences, thereby synthesizing a first strand cDNA from the mRNA by rever transcription reaction, (2) first strand cDNA reaction vesl which is synthesized on the solid support left within, the step of removing the reaction reagent from the reaction vesl, (3) a step of adding a polynucleotide quence consisting of one type of nucleotide to the 3 'end of first strand cDNA on the solid support, (4) above the cDNA polynucleotide quences are added, a cond DNA probe containing a quence complementary to a cond tag quence and the polynucleotide quence by hybridizing step of synthesizing the cond strand cDNA, (5) the cond strand cDNA synthesized on a solid support said method comprising performing DNA amplification reaction as a template.
铿锵
申请人：株式会社日立製作所
地址：東京都千代田区丸の内一丁目６番６号
国籍：JP
代理人：平木 祐輔，藤田 節足付
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