精确显微技术条件下构建脊髓损伤模型的脊髓离断状态

更新时间:2023-07-07 01:12:53 阅读：评论：0

李晓寅1，2，陈旭义2，涂悦2，刘英富2，徐云强3，杨晓青1，2，李瑞欣4，李娜5，李建国2

1天津中医药大学，天津市 300193；2武警后勤学院附属医院，天津市 300162；3天津医科大学总医院，天津市 300193；4军事医学科学院卫生装备研究所，天津市 300161； 5北京中日友好医院，北京市 100029

Spinal cord tranction in a spinal cord injury model established using accurate micrological technique

Li Xiao-yin 1, 2, Chen Xu-yi2, Tu Yue2, Liu Ying-fu2, Xu Yun-qiang3, Yang Xiao-qing 1, 2, Li Rui-xin4, Li Na 5, Li Jian-guo2

1 Tianjin University of Traditional Chine Medicine, Tianjin 300193, China; 2 Affiliated Hospital of Logistics University of Chine People’s Armed Police Forces, Tianjin 300162, China; 3 General Hospital of Tianjin Medical University, Tianjin 300193, China; 4 Institute of Medical Equipment, Academy of Military Medial Sciences, Tianjin 300161, China; 小清新美女5 China-Japan Friendship Hospital, Beijing 100029, China

摘要

背景：弥散张量成像是一项常用于大脑临床研究中的技术，但很少用于脊髓损伤的诊断或预后。
目的：采用显微技术建立大鼠脊髓损伤模型，用弥散张量成像技术评测脊髓离断情况，这为进一步的干预治疗提供良好的动物损伤模型。
方法：健康SD大鼠12只在精确显微技术下制备脊髓损伤模型，6只假手术组作为对照。用核磁弥散张量成像技术观察实验组大鼠造模后脊髓离断情况，用运动诱发电位和感觉诱发电位检测大鼠神经电生理改变情况，利用斜坡实验和BBB评分评价大鼠造模后的神经功能的变化。
结果与结论：实验组大鼠苏醒后双下肢全瘫、尾巴不能摆动、尿潴留；弥散张量成像图像显示其T10段脊髓完全离断；运动及感觉诱发电位波形较对照组均未引出；造模后1 d、造模后1，2，4周斜板试验角度均小于30°，BBB评分均少于10分，随时间延长，部分大鼠可见后肢刺激性反射，但无主动性功能活动，局部脊髓结构破坏严重。说明精确显微技术能成功构建大鼠脊髓损伤模型，并且在弥散张量成像图像上清晰可见T10段脊髓完全离断。

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

形容凉快的成语下月全文链接：

窗体顶端

关键词：实验动物；组织构建；脊髓损伤；显微技术；弥散张量成像；神经电生理；诱发电位；诱发电位；大鼠；动物模型；国家自然科学基金；

Abstract：

BACKGROUND: Diffusion tensor imaging is a common technique applied for clinical studies of the brain, but it is rarely ud for the diagnosis or prognosis of spinal cord injury.
OBJECTIVE: To establish a rat model of spinal cord injury using micrological techniques, and to evaluate spinal cord tranction with diffusion tensor imaging technology, thus providing a good animal model for further intervention.
METHODS: Twelve healthy Sprague-Dawley rats were applied to establish spinal cord injury models using preci microscopic techniques, and another six rats in the sham operated group rved as controls. Spinal cord tranction of experimental rats after modeling was obrved using diffusion tensor imaging. Motor evoked potentials and somatonsory evoked potentials were ud to detect electrical physiological changes of rats. Neurological function changes of rats were evaluated using slope experiments and Basso, Beattie and Bresnahan scores.
RESULTS AND CONCLUSION: After experimental rats regained consciousness, their lower extremities exhibited complete paralysis, the tails cannot swing, accompanying urinary retention. Diffusion tensor imaging displayed the T10 gment of spinal cord was completely trancted. Motor and nsory evoked potential waveform were not drawn compared than control group. At 1 day, 1 week, 2 weeks, 4 weeks after operation, the test angle of slope experiments was less than 30° and Basso, Beattie and Bresnahan score was less than 10 points; as the time prolonged, lower limb irritating reflections of some rats were visible, but no initiative functional activity was found, local spinal cord structure were verely damaged. Preci microscopic techniques can successfully establish spinal cord injury model in rats, and diffusion tension imaging clearly visualizes the complete tranction of the T10 spinal cord.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words： spinal cord injuries; models, animal; diffusion tensor imaging; electrophysiology; evoked potentials;

窗体底端

中图分类号: 得不到的幸福	R318

斯年如你基金资助: 武警后勤学院创新团队科学基金资助(WHTD201306)；国家自然科学基金资助项目( 11102235，31200809，81271392，81101362)；天津市应用基础及前言技术研究计划重点项目资助(12JCZDJC24100)；天津市应用基础及前言技术研究计划面上项目(10JCYBJC13500)

通讯作者: 李建国，博士，教授，主任医师，硕士生导师，武警后勤学院附属医院，天津市 300162

作者简介: 李晓寅，男，1986年生，汉族，天津市人，在读硕士，主要从事神经病学方面的研究。

澳大利亚大陆

引用本文:

李晓寅,陈旭义,涂悦等. 精确显微技术条件下构建脊髓损伤模型的脊髓离断状态[J]. 中国组织工程研究, 2014, 18(27): 4282-4286.

大满贯什么意思

Li Xiao-yin,Chen Xu-yi,Tu Yue et al. Spinal cord tranction in a spinal cord injury model established using accurate micrological technique[J]. Chine Journal of Tissue Engineering Rearch, 2014, 18(27): 4282-4286.

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