皇帝顺序南京农业大学学报2020,43(5) ：910-918
Journal of Nanjing Agricultural University http ://nauxb.njau.edu DOI ： 10.7685/jnau.201906050王换换，刘颖，李帅，等.肾素-血管紧张素系统2条通路在油酸致大鼠BRL-3A 细胞非酒精性脂肪肝中的作用[J ].南京农业大学学报， 2020，43(5):910-918.
WANG Huanhuan , LIU Ying , LI Shuai , et al. Role of two pathways of renin-angiotensin system in oleic acid-induced non-alcoholic fatty liver dia in mou BRL-3A cells [ J ]. Journal of Nanjing Agricultural University ,2020,43(5) ：910-918.
肾素-血管紧张素系统2条通路在油酸致大鼠
复活节彩蛋BRL-3A 细胞非酒精性脂肪肝中的作用
王换换，刘颖，李帅，何晟，朱斌，张源淑*
（南京农业大学农业农村部动物生理生化重点开放实验室，江苏南京210095）
摘要：［目的］通过建立油酸诱导的大鼠肝脏间质BRL-3A 细胞非酒精性脂肪肝（NAFLD ）损伤模型，探讨BRL-3A 细胞中 RAS 的ACE/AngH/ATIR 和ACE2/Ang-l-7/Mas 这2条通路相互负向调节与NAFLD 细胞损伤的关系。［方法］采用不同 浓度油酸（0.025、0.05、0.1和0.2 mmol-L -1 ）处理BRL-3A 细胞24 h ，通过检测细胞活力、细胞内甘油三酯（TG ）含量、丙氨酸 氨基转移酶（ALT ）活性、天门冬氨酸氨基转移酶（AST ）活性以及油红染色确立NAFLD 细胞模型条件，然后选取低、中、高3个 浓度（0.025,0.1和0.2 mmol-L -1）油酸作用于细胞24 h ，用ELISA 法检测细胞上清液中血管紧张素H （ AngH ）、血管紧张素 1-7（ Ang1-7）水平；Western blot 分析细胞内血管紧张素转化酶（ACE ）、血管紧张素转化酶2（ ACE2）、血管紧张素H 受体1 （AT1R ）、Mas 受体（MasR ）蛋白水平撕皮尔曼相关分析法分析油酸浓度与细胞内ACE 、ACE2表达水平的相关性。［结果］ 1）用0.1 mmol-L -1油酸处理BRL-3A 细胞24 h ，成功建立BRL-3A 细胞NAFLD 模型。2）油酸处理24 h 后，BRL-3A 细胞出 现脂滴沉积，在0.025 mmol-L -1油酸（低浓度）组,ACE2、MasR 表达水平和Ang1-7水平有升高趋势,AngH 水平与AT1R 表 达水平下降,ACE/ACE2值下降；0.1 mmol-L -1油酸（中浓度）组ACE 、AT1R 表达水平显著升高，其他RAS 成员变化不明显； 0.2 mmol-L -1油酸（高浓度）组,ACE2、MasR 表达水平和Ang1-7水平下降,AngH 水平和ACE 、AT1R 表达水平及ACE/ACE2 值升高。3）斯皮尔曼相关分析显示，油酸浓度、血液相关生化指标（TG 含量、AST 和A
LT 活性）和脂滴数及脂滴面积与 ACE2表达水平和Ang1-7水平负相关，而与ACE 表达水平和AngH 水平正相关。［结论］BRL-3A 细胞中的2条轴共同参与 了油酸致BRL-3A 细胞NAFLD 损伤的发生和发展过程。低浓度油酸时,ACE2介导的Angl-7/MasR 通路占优势；高浓度油 酸处理细胞损伤加重,ACE 介导的AngH/ATIR 通路占主导地位。ACE2与油酸致细胞炎性损伤的程度呈显著负相关关系。 关键词：血管紧张素系统（RAS ） ；BRL-3A 细胞；非酒精性脂肪肝病（NAFLD ）细胞模型
用字组词
中图分类号:S852.2 文献标志码：A 文章编号：1000-2030（ 2020） 05-0910-09Role of two pathways of renin-angiotensin system in oleic acid-induced
中国历史年代表non-alcoholic fatty liver dia in mou BRL-3A cells WANG Huanhuan ,LIU Ying ,LI Shuai ,HE Sheng ,ZHU Bin ,ZHANG Yuanshu *(Key Laboratory of Animal Physiology and Biochemistry and Rural Affairs , Ministry of Agriculture ,
Nanjing Agricultural University ,Nanjing 210095,China )
少儿爵士舞视频Abstract : [ Objectives ]The relationship between the negative regulation of ACE/AngH/ATIR and ACE2/Ang-l-7/Mas pathways of RAS and the damage of non-alcoholic fatty liver dia ( NAFLD ) in mou liver stromal cells ( BRL-3A ) was investigated by establi shing injury model of NAFLD in mou BRL-3A cells induced by oleic acid. [ Methods ] BRL-3A cells wer
天生黑怎么变白
坠的成语e treated with different concentrations (0.025,0.05,0.1 and 0.2 mmol • L -1 ) of oleic acid for 24 h. The cell viability assay , intracellular TG content , ALT and AST activities and oil red staining were ud to confirm the NAFLD cell model. Then , three oleic acid concentrations ( 0.025,0.1 and 0.2 mmol *L -1) were applied to the cells for 24 h for subquent experiments. The content of Ang H and Ang1 -7 in the supernatant was detected by ELISA. The expression levels of angiotensin converting enzyme ( ACE ) ,angiotensin converting enzyme ( ACE2) ,angiotensin typel receptor ( AT1R ) and Mas receptor ( MasR ) in the cells were analyzed by Western blot. Meanwhile ,Spearman correlation analysis was ud to analyze the correlation between different concentrations of oleic acid treated for 24 h and the levels of intracellular ACE and ACE2. [ Results ] 1) Using 0. 1 mmol • L -1 oleic acid to treat cells for 24, the NAFLD model of BRL-3A cells was successfully established. 2)Using oleic acid to treat cells for 24, lipid droplets were deposited in BRL-3A cells. The expression levels of ACE2, MasR and Ang1 -7 content incread with 0.025 mmol • L -1 oleic acid , however , the expression level of AT1R , Ang H content and the 收稿日期：2019-06-25
基金项目：国家自然科学基金项目（30871838）；江苏高校优势学科建设工程资助项目（PAPD ）
作者简介：王换换，助理实验师。*通信作者：张源淑，教授，博士，主要从事动物营养和机能生物化
糯米八宝饭的做法
学研究,E-mail ：zhangyuanshu@ njau.edu 。
