USDA Pain Levels:
USDA Category B USDA Category C USDA Category D USDA Category E
Breeding or Holding Colony Protocols No more than momentary or
slight pain or distress and no
u of pain-relieving drugs, or
no pain or distress. For
example: euthanatized for
tissues; just obrved under
normal conditions; positive
reward projects; routine
procedures; injections; and
blood sampling.
Pain or distress appropriately
remain用法
relieved with anesthetics,
analgesics and/or tranquilizer
drugs or other methods for
relieving pain or distress.
Pain or distress or potential
pain or distress that is not
relieved with anesthetics,
analgesics and/or tranquilizer
drugs or other methods for
relieving pain or distress.
Examples Examples Examples
1.Holding or weighing
animals in teaching
or rearch
activities.
2.Injections, blood
collection or
catheter
implantation via
superficial vesls.
3.Tattooing animals.
4.Ear punching of
rodents.
5.Routine physical
examinations.
6.Obrvation of
animal behavior.
7.Feeding studies,
which do not result
in clinical health
problems.
8.AVMA approved
humane euthanasia
procedures.
9.Routine agricultural
husbandry
procedures.
10.Live trapping.
11.Positive reward
projects.
1.Diagnostic
procedures such as
laparoscopy or
needle biopsies.
2.Non-survival
surgical procedures.
3.Survival surgical
procedures.
4.Post operative pain
or distress.
5.Ocular blood
collection in mice.
6.Terminal cardiac
blood collection.
7.Any post procedural
outcome resulting in
evident pain,
discomfort or
distress such as that
associated with
decread appetite/
activity level,
adver reactions,
to touch, open skin
lesions, abscess,
lameness,
conjunctivitis,
corneal edema and
photophobia.
8.Exposure of blood
vesls for catheter
implantation.
9.Exsanguination
under anesthesia.
10.Induced infections
or antibody
情实
production with
appropriate
anesthesia and
post-op/post-
ps怎么加阴影
procedure analgesia
when necessary.
1.Toxicological or
microbiological
testing, cancer
rearch or
infectious dia
rearch that
requires
continuation until
clinical symptoms
are evident or death
occurs.
2.Ocular or skin
irritancy testing.
3.Food or water
deprivation beyond
that necessary for
ordinary pre-surgical
preparation.
4.Application of
noxious stimuli such
as electrical shock if
the animal cannot
avoid/escape the
stimuli and/or it is
vere enough to
cau injury or more
补牙用什么材料好
than momentary
pain or distress.
5.Infliction of burns or
trauma.
一修6.Prolonged restraint.
7.Any procedures for
which needed
analgesics,
tranquilizers,
datives, or
anesthetics must be
withheld for
justifiable study
purpos.
8.U of paralyzing or
immobilizing drugs
for restraint.
9.Exposure to
abnormal or
extreme
environmental
conditions.
10.Psychotic-like
behavior suggesting
a painful or
distressful status.
凌志军
中国古代四大凶兽11.Euthanasia by
procedures not
approved by the
AVMA.
Guidelines for determining USDA classification in protocols involving tissue collection before/after euthanasia and/or animal perfusion:
If an animal will be euthanatized by an approved physical or chemical method of euthanasia solely for the collection of tissues (after the animal's death), the procedure should be classified as USDA C.
If an animal will be anesthetized so that non-vital tissues can be collected (liver or skin biopsy), and the animal will then be allowed to recover, the procedure should be classified as USDA D (survival surgery).
If an animal will be anesthetized so that non-vital tissues can be collected (liver or skin biopsy, etc.);
and the animal will then be euthanatized, the procedure should be classified as USDA D (non-survival surgery). In this scenario, it is necessary to justify why the animal couldn't be euthanatized (USDA category C) rather than anesthetized.
If an animal will be anesthetized so that vital tissues can be collected (heart, both kidneys or lungs, whole liver, etc.), the animal will obviously succumb to the procedure. To determine whether this will be euthanasia or non-survival surgery, we must consider the definition of euthanasia. A critical
component of this definition is "rapid unconsciousness followed by loss of cardiac, respiratory and brain function". Bad on this definition, procedures which require tissue manipulation or other
prolonged techniques prior to the animals death (more than a few minutes) should be classified as non-survival surgery (USDA D). Similarly, if an animal will be anesthetized so that the tissue can be collected in the "freshest" possible state (i.e. heart) and the tissues will be rapidly excid, the
procedure should be classified as euthanasia (USDA C). (Note: In this scenario, it is difficult to justify why the animal couldn't be euthanatized rather than anesthetized.)
If an animal will be anesthetized so that it can be chemically perfud, the same "test of time"
applies (i.e.: long, technical manipulations should be classified as USDA D; while rapid intravascular injection of the perfusate without other manipulations should be classified as USDA C).碳粉打印机
NOTE: Becau the USDA classification system is bad on the "potential for pain, distress or
discomfort," the anesthetic/euthanasia drug do becomes a critical concern. For example, if a known "euthanasia do" of pentobarbital will be administered, drug irreversibility is assumed. Thus, once the animal is confirmed to be in an anesthetic plane (toe pinch respon, etc.), tissues can be
collected/ procedures can be performed without the concern about what the animal will be
perceiving. This procedure would then be classified as USDA C. The Committee recommends using a euthanizing do whenever possible. Other methods may be appropriate with proper scientific
justification.
