Expression, characterization, and site-directed mutagenesis of UDP-glycosyltransfera UGT88A1 from Arabidopsis thaliana
期刊名称： Bioengineered Bugs
作者： Jingyuan Weng,Liangliang Chen,Yinchu Cheng,Yan Li,Honghua Jia,Hua
Zhou,Ping Wei
作者机构： College of Biotechnology and Pharmaceutical Engineering, Nanjing
小学数学思维
Tech University, Nanjing, China
年份： 2019年惊蛰
期号： 第1期
中国拳击关键词： Quercetin;quercetin-4′-O-glucoside;site-directed mutation;uridine
diphosphate glycosyltransfera 88A1编童话故事作文三年级
爨文化冰草菜摘要：Quercetin-4′- O -glucoside is one of the major quercetin derivatives in the mature red onion bulb. It has an adjuvant effect on allergies, asthma, arthritis, and cancer. The prent study aimed to u uridine diphosphate glycosyltransfera
88A1 (UGT88A1) from Arabidopsis thaliana to achieve the enzymatic synthesis of quercetin-4′- O -glucoside from quercetin. The results showed that UGT88A1 was most active at pH 9.0. The optimum temperature of UGT88A1 for synthesizing quercetin-4′- O -glucoside was 45°C, which was a little lower than that for synthesizing quercetin-3- O -glucoside (50°C). One mutant, V18R, of UGT88A1 was obtained by site-directed mutation and showed a greater affinity ( K m 0.20 mM) and twice the enzyme activity (552.3 mU/mg) towards quercetin compared with the wild-type enzyme (0.36 mM and 227.6
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