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补牙用什么材料好>反蛙泳Grapevine virus A variants of group II associated with Shiraz dia in South Africa are prent in plants affected by Australian Shiraz dia, and have also been detected in the USA 期刊名称: PLANT PATHOL
作者: Goszczynski,DE,Habili
作者机构: [Goszczynski
年份: 2012年
期号: 第-期
冬季钓鲫鱼饵料
关键词: Grapevine virus A;RT-PCR;Shiraz dia;single-strand conformation
polymorphism;variants
卷面分析怎么写
摘要:Primers were designed for RT-nested PCR amplification of the highly
variable 293-nt fragment from the 5' terminal part of the Grapevine virus A (GVA)
replica gene, specific to South African variants of molecular groups I and II. This
new technique, along with RT-PCR for simultaneous amplification of variants of
groups I, II and III, as well as cloning of amplicons, single-strand conformation
polymorphism (SSCP) analysis of clones and quencing, were ud to investigate小强盗
the populations of variants infecting 16 local Shiraz grapevines with different Shiraz
dia (SD) status. The techniques were also ud to study variants in GVA-infected
grapevines from Australia and the USA. The Australian grapevines included ven垃圾文件清理
plants of cvs Shiraz and Merlot affected by Australian Shiraz dia (AuSD), and
one plant of cv. Crimson
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