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KREBS-HENSELEIT BUFFER
With 2000 mg/L Gluco, Without Calcium Chloride and Sodium Bicarbonate
Product Number K 3753
穆斯林朝圣Product Description
Krebs-Henleit buffer was developed in the early
1930's by Hans Krebs and Kurt Henleit. This
modification of Ringer's solution was ud to maintain
liver tissue during experiments that led Krebs to
postulate the urea cycle. The formula offered by Sigma
has been modified by the addition of 2 g/L of gluco as
an energy source for cell maintenance, and by the
omission of calcium chloride.
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Components g/L
D-Gluco 2.0
Magnesium Sulfate [Anhydrous] 0.141
Potassium Phosphate Monobasic 0.16
Potassium Chloride 0.35 Sodium Chloride 6.9 Precautions and Disclaimer For In Vitro Diagnostic U MSDS Available. Caution - Substance not yet fully tested (EU) Preparation Instructions Powdered salts are hygroscopic and should be protected from moisture. The entire contents of each package should be ud immediately after opening. Preparing a concentrated salt solution is not recommended as precipitates may form. Supplements can be added prior to filtration or introduced aptically to sterile salt solution. 1. Measure out 90% of final required volume of water. Water temperature should be 15-20 °C. 2. While gently stirring the water, add the powdered medium. Stir until dissolved. Do NOT heat. 3. Rin original package with a small amount of water to remove all t
races of powder. Add to solution in step 2. 4. To the solution in step 3, add 0.373 g of calcium
chloride dihydrate for each liter of solution being prepared. Stir until dissolved.
5. To the solution in step 4, add 2.1 g sodium bicarbonate or 28 ml of sodium bicarbonate solution [7.5% w/v] for each liter being prepared. Stir until dissolved.
6. While stirring, adjust the pH of the medium to 0.1-0.3 pH units below the desired pH since it may ri during filtration. The u of 1N HCl or 1N NaOH is recommended.
民事诉讼状模板7. Add additional water to bring the solution to final volume.
8. Sterilize immediately by filtration using a membrane with a porosity of 0.22 microns.
9. Aptically dispen medium into sterile container. Storage/Stability Store the dry powdered salts at 2-8 °C under dry conditions and liquid medium at 2-8 °C in the dark. Deterioration of the powdered medium may be recognized by any or all of the following: [1] color change, [2] granulation/clumping, [3] insolubility. Deterioration of the liquid medium may be recognized by any or all of the following: [1] pH change, [2] precipitate or particulates, [3] cloudy appearance [4] color change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears e
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xpiration date. Procedure Materials Required but Not Provided: Water for tissue culture [W 3500] Sodium Bicarbonate [S 5761] or
Sodium Bicarbonate Solution, 7.5% [S 8761] Calcium Chloride, Dihydrate [C 7902]
1N Hydrochloric Acid [H 9892] 1N Sodium Hydroxide [S 2770]
Medium additives as required
Product Profile
Appearance Off-white powder Moisture content ≤2.0% Solubility Clear solution at 1x
霸气诗句
pH at RT 4.9 ± 0.3 [without sodium bicarbonate]
pH at RT 7.5 ± 0.3 [with sodium bicarbonate]临沂电视塔
酥油茶
Osmolality 249
mOsm/kg
H2O ± 5% [without sodium bicarbonate]
Osmolality 289
mOsm/kg
H2O ± 5% [with sodium bicarbonate]
Endotoxin ≤1.0 EU/ml at 1x Key Element Analysis Consistent with formula
by ICAP Biological Performance Characteristics
The ability of this balanced salt solution to maintain the structural integrity of cells in culture is evaluated using appropriate cell lines.
References
1. Krebs, H. A. and Henleit, K. (1932).
Untersuchungen über die Harnstoffbildung im
Tierkörper. Hoppe-Seyler's Zeitschrift für Physiol.
Chemie. 210, 33-66.
JWM 5/29/2003
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchar must determine the suitability of the product(s) for their particular u. Additional terms and conditions may apply. Plea e rever side of the invoice or packing slip.
