Molecular epidemiology with subtype analysis of Cryptosporidium in calves in Belgium 期刊名称: Parasitology圉怎么读音
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作者: GEURDEN, T.,BERKVENS, D.,MARTENS, C.,CASAERT, S.,VERCRUYSSE,
J.,CLAEREBOUT, E.
年份: 2007年
台球练习期号: 第14期
国家安全宣传日雪上加霜的近义词关键词: Cryptosporidium; calf; molecular epidemiology; gp60 subtype; Belgium真假小叶紫檀
稻花摘要:The prevalence ofCryptosporidiumin calves younger than 10 weeks was estimated in a cross-ctional epidemiological study on 100 dairy (n=499) and 50 beef (n=333) farms in East Flanders (Belgium), using a previously evaluated immunofluorescence assay (Merifluor). The calf prevalence was 37% (95% Probability Interval (PI): 7–70%) in dairy calves and 12% (95% PI: 1–30%) in beef calves. To elucidate the genetic diversity, theCryptosporidium18S ribosomal DNA and the 70kDa heat s
hock protein gene were targeted. In the majority of the samplesC. parvumwas prent, althoughC. boviswas also identified, all but one in calves older than 1 month. The porcine-specificC. suiswas identified in 1 beef calf. Subtyping ofC. parvumpositive isolates by quence analysis of the 60kDa glycoprotein gene indicated the prence of 4 allele IIa subtypes,
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