This Data Sheet Contains Important Information About The Product.自制皮冻的家常做法
Solid Pha Microextraction Fiber Asmblies
SUPELCO Bellefonte, PA
T794123M
©1999 Sigma-Aldrich Co.
(contd. on back of sheet)
◆
This fiber is more durable than the others listed. It contains no epoxy ●
Special 2cm length. Does not contain spring.
Non-bonded phas are stable with some water-miscible organic solvents, but slight swelling may occur. NEVER u or rin with nonpolar organic solvents.Bonded phas are stable with all organic solvents. Slight swelling may occur when ud with some nonpolar solvents.Note: For solvent/fiber compatibility, refer to Supelco application notes and bulletins that describe SPME/HPLC analys.Note: Fibers for autosampler holders (57331 & 57347-U) can be ud with manual holder (57330-U).
微信怎样建群CAUTION: CHLORINATED SOLVENTS MAY DISSOLVE THE EPOXY THAT HOLDS THE FIBER.
USE EXTRA CAUTION WHEN HANDLING PDMS/DVB AND CW/DVB FIBERS. THE COATING CAN BE INADVERTENTLY STRIPPED OFF.
巳时属什么Solid Pha Microextraction (SPME) Technology licend exclusively to Supelco. US Patent #5,691,206: European Patent #0523092.Carboxen and StableFlex are registered trademarks of Sigma-Aldrich Co. Carbowax is a registered trademark of Union Carbide Corp.
PDMS
100µm 2-10280°C 200-280°C 250°C 0.530µm 2-11280°C 200-280°C 250°C 0.57µm 2-11340°C 220-320°C 320°C 1PDMS/DVB 65µm 2-11270°C 200-270°C 250°C 0.5Polyacrylate 85µm 2-11320°C 220-310°C 300°C 2CAR/PDMS 75µm 2-11320°C 250-310°C 300°C 1-2CW/DVB
65µm 2-9260°C 200-250°C 220°C 0.5DVB/CAR/PDMS
50/30µm 2-11270°C 230-270°C 270°C
1
Fiber Asmbly Ud With SPME Holder 57330-U
(For Manual U)
P000812
P000813
黄山的松树P000816
P000815
796-0602
Plain Hub
Attaching the Fiber Asmbly to the Holder To begin to u SPME, you need to attach the fiber
asmbly to the SPME holder. Follow the simple
steps.
1.Unscrew the black cylinder like depth gauge from
the holder.
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2.Unscrew the threaded end-cap on the end of the
冷清反义词holder.
3.Push the black plunger forward through the Z-slot
on the ba of the holder to expo the end of the
plunger. Note internal threads inside of the plunger
will accept the threaded fiber asmbly.
4.Screw the fiber asmbly into the end of the
plunger.
5.Retract the plunger by pulling it back through the
Z-slot and slide the threaded end-cap over the
needle. Screw the threaded end-cap tightly onto
the end of holder.
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6.Screw the black depth gauge onto the end of the
holder over the threaded end-cap.
7.Test the holder/fiber by pushing the plunger forward until the
fiber is expod from the protective needle. Stop at the Z-slot to hold the fiber in the expod position during sampling and injection in the GC.
8.To retract the fiber, move the plunger out of the Z-slot and draw
it back.
The fiber asmbly is attached the same way to the autosampler holder. Remove hexagonal nut and push black plunger down to expo threaded port for fiber asmbly attachment. No spring is ud with the autosampler fiber asmblies becau the autosampler controls the movement of the plunger and fiber. Autosampler fiber asmblies can be ud with manual holders but the manual fiber asmblies cannot be ud with the autosampler holders becau of the spring.
For GC U
Choosing a Fiber
As a first step, identify the molecular weight range of the analytes to be extracted. Higher molecular weight compounds desorb easier from the surface of the 7µm or 30µm PDMS absorption fiber coatings. Smaller molecules are retained in the pores of the fibers containing adsorbents in the coating; e.g. Carboxen, divinylbenzene particles. Further, refine your choice by matching the fiber coating relative to analyte polarity.
StableFlex SPME fibers are coated on a flexible fud silica core. The coating partially bonds to the flexible core which results in a more stable coating and a less breakable fiber. The extraction lectivity of StableFlex fibers may be slightly different from the same coating on a standard fud silica core.
23 gauge needles are specifically designed to meet the specifi-cations of Merlin Microal ptum system. The needle is also suitable for other ptumless als.The 23 gauge needles are not recommended for u with standard silicone pta as they verely core the ptum.
2cm fibers are available in the StableFlex DVB/CAR/PDMS fibers. They do not contain springs and must be manually retracted. They are not recommended for autosampler u.
Conditioning Instructions
Set the injection port temperature according to Table 1 on this sheet. Install a column into the GC and adjust carrier gas to the desired flow rate. U a carrier gas purifier to reduce oxygen in the carrier gas to minimize oxidation of the fiber surface during conditioning. If a splitless/split injection port is ud, open the splitter vent. Inrt the SPME needle into the GC injection port, expo the fiber, and condition for the recommended time. Longer than recommended conditioning times will not hurt the fiber. After conditioning is completed, retract the fiber and remove the needle from the injection port. Condition the column for an additional 30minutes at the upper temperature of the program.
THE POLYACRYALATE FIBER WILL TURN BROWN AS A RE-SULT OF CONDITIONING. THIS WILL NOT AFFECT THE PER-FORMANCE OF THE FIBER.
Blank Analysis
After conditioning the fiber, t the injection port to the desired operating temperature and cool the GC oven to 50°C or lower. Set the detector attenuation at the nsitivity typical for the desired analysis. Clo the split injection port valve, inrt the SPME needle and start the GC temperature pr
ogram. Desorb the fiber for 5 minutes in the injection port while programming the GC. Retract the fiber and remove the needle after 5 minutes and obrve the chromatogram when the program is completed. There may be some extraneous peaks from the previous extraction or exposure of the fiber since the initial conditioning of the fiber. Redesorb the fiber, the intensity of the peaks should decrea after the cond desorption step. Plea contact our technical rvice department for further assistance if the background remains too high after following the steps.
Cleaning Fibers
You can clean verely contaminated fibers by one of two meth-ods. First, you can heat the fiber to 20°C below its recommended maximum temperature for one hour to overnight. Check the table of fibers to determine the recommended maximum temperature.
A cond alternative is to rin the fiber by soaking it in a water miscible solvent for one hour, then thermally desorb it for thirty minutes. NEVER RINSE A FIBER IN CHLORINATED SOLVENTS. The solvents will damage the fibers.
Fiber Selection
The fiber asmblies recommended for HPLC are crimped rather than u epoxy to attach the fiber in the fiber asmbly. Four fibers are recommended for u with HPLC solvents: 100µm PDMS, 60µm PDMS/DVB, 50µm Carbowax/templated resin, and 85µm polyacrylate.
For HPLC U
Conditioning Instructions
The effects of different solvents on an SPME fiber will vary. It is best to condition the fiber with the mobile pha or solvent to which it will be expod. Referring to the instructions for using the SPME/ HPLC interface, expo the fiber in the interface. Switch the valve from the LOAD position to the INJECT position, allowing the mobile pha to pass through the interface (dynamic mode). If a gradient is ud, run the gradient through the entire cycle. The fiber should be conditioned for a minumum of 30 minutes.
If the fiber is desorbed in a solvent different than the mobile pha, switch the valve to the LOAD position, fill the valve desorption chamber with the solvent, and allow the fiber to soak in the solvent (static mode) for a minimum of 15 minutes. The background can be checked by switching the valve to the INJECT mode and monitoring baline throughout the run cycle. The fiber may need additiona
l conditioning if the background is not sufficiently clean. Minimal cleaning is usually required, but cleaning time often depends on the type of detection and nsitivity.
Solvent Effects on Extraction of Samples
If organic solvents are prent in the fiber, they may affect the extraction of the next sample. As a precaution, if the fiber has been desorbed in organic solvents, allow the fiber to dry for veral minutes prior to starting the next extraction. You may determine that the drying step is not necessary for particular samples. The effect of solvents on the fiber can be determined by comparing results of two extractions — one with a dried fiber, and the other
with a non-dried fiber. G000351
