The Nijmegen modification of the Bethesda assay fo

更新时间:2023-06-11 18:51:12 阅读:21 评论:0

The Nijmegen modification of the Bethesda assay for factor VIII:C inhibitors: improved specificity and
reliability.
期刊名称: Thrombosis & Haemostasis
母子公司作者: VERBRUGGEN,B
年份: 1995年
关于荷花的诗期号: 第2期
性健康教育关键词: Humans;Hemophilia A;Factor VIII;Antibodies;Immunoassay;Logistic
Models;Sensitivity and Specificity;Reproducibility of Results;Predictive Value of
Tests;Hydrogen-Ion Concentration
上升三角形>心出发摘要:Antibodies against factor VIII coagulant activity can appear in haemophiliacs who are treated wit
风的形成原因请给我一双翅膀h factor VIII preparations but also spontaneously in non-haemophiliacs. The Bethesda assay is the most commonly ud method to detect the antibodies, but it lacks specificity especially in the lower range resulting in unreliable data. Two modifications are propod and tested to resolve the imperfections: 1. Buffering the normal plasma ud in the assay- and control mixture with 0.1 M imidazole to pH 7.4. Replacing the imidazole buffer in the
control mixture by immunodepleted factor VIII deficient plasma. The modifications allow better discrimination between positive and negative samples and improve reliability.
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