会计工作简历专利名称:Methods for the Cryoprervation of Mammalian Cells
发明人:Xiaoming He,Thomas L. Toth,Mehmet Toner,Jon Edd
申请号:US12226300
申请日:20070412
公开号:US20090305224A1
公开日:
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小清新情侣头像摘要:The prent invention features novel methods for the cryoprervation of mammalian cell that combine the advantages of the slow-freezing and vitrification approaches while avoiding their shortcomings. Generally, the methods include the u of a capillary tube made of a thermally conductive wall material and a thin wall such that the ratio of the thermal conductivity of the wall material to the wall thickness is at least
1,000-500,000. The solution is then expod to temperatures equal to or less than −80°C. and the vitrification solution containing the mammalian cells is cooled at a rate equal to or greater than 30,000-100,000,000° C./minute. The exposure of the capillary tube with a thermally conductive and thin wall allows for vitrification of the solution in the abnce of ice formation. Cryoprotectants can also be added to the vitrification solution to further prevent ice formation.
申请人:Xiaoming He,Thomas L. Toth,Mehmet Toner,Jon Edd
地址:Winchester MA US,Sudbury MA US,Wellesley MA US,Arlington MA US
抗战胜利的意义>宝宝咳嗽老不好怎么办呀国籍:US,US,US,US
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