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Manipulation and Misconduct in the Handling of Image Data
The past few years have en a small number of celebrated cas of scientific fraud that have found their way into the general media. Many more examples of inappropriate data handling have come across the editorial desks of virtually every scientific journal.The have focud editors’attention on inappropriate data handling and fraudulent image manipulation.The Plant Cell and Plant Physiology are no exceptions.Two decades ago,the practicalities of image handling meant that the boundaries were well-defined between what was acceptable and what was not;the darkroom skills needed pod a significant technical barrier to inappropriate manipulation of image data,particularly manipulation done without the intention to deceive but simply to“clean up”the image.The ethical boundaries are as clear-cut today as they were a quarter century ago,but many of the technical barriers to inappropriate manipulation have all but disappeared with the advent of digital image acquisition,storage,and handling.Adobe Photoshop was introduced in1990for Macintosh and in1992for personal computers;its widespread application,and the broader acceptance of digital formats during this past decade,have simplified greatly the tasks of image preparation.They also mean that much less skill is needed to manipulate images.Indeed,a common problem arising from digital formats is that many scientists inadv
ertently manipulate their image data,often in ways that result in the loss of important information,to make their data look as good as possible. The Journal of Cell Biology performed a detailed study over the past decade and,commendably,has shared this information publicly.The study found that10%of articles accepted for publication included inappropriate manipulations of image data that contravened journal policy,even if they did not alter the conclusions drawn from the data(e International Society of Managing and Technical Editors,2013).A surprisingly large number of the authors appeared unaware that they had handled image data inappropriately and,in many cas,were not conscious of the ethical issues and conquences of their actions.As editors, how do we maintain ethical standards in publishing?And,as scientists,how do we educate our students and support our peers to understand what is(and what is not)acceptable practice when handling image data?
It is esntial to recognize that digital images are data,in fact arrays of numerical data,and must be treated as such.As scientists,we assume that images will not have been altered in any way that affects the visual impression;the quantitative and qualitative relationships within images(data arrays)must be maintained.If the relationships are altered,then such alterations must be fully documented and explained.There are two defining principles behind the expecta-tions:(1)We expe
ct honesty and transparency in scientific reporting, and(2)We expect the scientist,as author,to understand the conquences of processing image data to ensure that any trans-formations are quantitatively rigorous and comply with ethical standards.There are a few simple rules to follow in meeting the expectations(e Rossner and Yamada,2004;North,2006;and Cromey,2010).
1.Raw image data must be saved and archived intact and without旅游策划
alteration as part of good laboratory practice.Processing of digital images should be done on a copy of the image datafile, not on the original.Retaining raw image data is important becau they rve as the standard against which thefinal image can be compared,and they ensure a route for recovery should
a mistake be made during processing.We recommend that
image data be saved in TIF format.JPEG compression affects the resolution of the image,and information is lost in the process of conversion.
2.Simple adjustments,applied uniformly,to the entire image are
generally acceptable.Changes to brightness,contrast,and color balance fall into this category becaus
快乐的春节作文e they affect the image in a linear fashion.However,it is not acceptable to adjust brightness or contrast levels to such an extent that image data are truncated or lost(giving a white or black background;
e Figure1B).Such changes may give a clearer picture of bands which are“of interest”in a gel,but they will mask background,including information that is important for quanti-fication and validation.We will not accept image data that are procesd in this way.
3.Cropping and resizing an image is usually acceptable,but both
may on occasion be construed as inappropriate manipulation.
If cropping,ask whether your motivation is to improve the composition of the image or to hide something that complicates interpretation.The former reason is acceptable;the latter is not.
4.Digitalfiltering of an image is not encouraged becau it can
easily mask important information.Mostfilters u mathemat-ical functions that are nonlinear.There are circumstances in which digitalfiltering is a necessary part of the experimental methodology.If so,filter processing must be clearly justified and documented in thefigure legend or under Methods.S
uch documentation should include reference to the software version and specification of thefilters and any special ttings that were ud.
5.Combining images is acceptable only if it is clear to the reader
that the images are from parate sources.It is acceptable to combine the images of two similar gels or two parts of the same gel in onefigure,but only if a visible gap is left between the images,or the images are parated and each surrounded by
a box.It is not acceptable to splice two gel images together so
that they appear to be adjacent tracks from a single gel.
6.Selective alteration or processing of one region of an image is not
acceptable.Such manipulations include“cloning”or copying objects or ctions within or between images and“smudging,”
8月用英语怎么说blurring,blending,and other manipulations that are applied
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locally within an image.Common examples (e Figure 1A)involve ctions of an image that have been cloned or blended to clean up a dirty preparation or to mask an unwanted blemish.Such manipulations constitute inappropriate handling at best and are unethical.If the data require such processing,repeat the experiment.
7.When comparing digital images,it is important that each has been acquired under identical conditions,and any postacqui-sition image processing must be applied identically.If the background or color balance must be adjusted among images within a group,this must be acknowledged in the figure legend or under Methods (e Figure 1B).Quantitative analysis of images should always be performed on uniformly procesd
image data,and the data should be calibrated to a known standard.Most instruments,including fluorescent microscopes,are prone to fluctuations and drift over time,so it is advisable to include appropriate internal standards as checks against such changes.
8.Image data should be documented both with reprentative images as well as with quantitative sta
tistical analysis of sufficient numbers of experiments.It should be lf-evident that experiments that include image data should be repeated and the data analyzed for significance.We expect conclusions drawn from image data to be justified bad on their quantitative as-ssment,not on anecdotal obrvations.As editors,we have a responsibility to the readers and authors of Plant Physiolog y and The Plant Cell to ensure that what we publish is sound scientifically and meets the highest ethical standards.We can help authors become aware of data mishandling and the ethical conquences of inappropriate manipulations,and address the probable 10%of articles falling into the category of data handling that is simply misguided or ethically ignorant.Most inappropriate data handling is relatively easy to spot and is often flagged by reviewers.From an editorial and educational standpoint,it is always best to identify and deal with such instances before an article is accepted.To this end,Plant Physiology and The Plant Cell will now have available the facility to analyze cas of suspect mishandling using the forensic tools ud by The Rockefeller University Press journals,including The Journal of Cell Biology .We are confident that the tools will give our editors the resources they need to handle problems of inappropriate data handling when questions ari.We hope,too,that our approach to the issues will help strengthen the scientific community and the reliability of the data we publish.
Cathie Martin Editor-in-Chief The Plant Cell Mike Blatt Editor-in-Chief Plant Physiology
REFERENCES浙江特色美食
Cromey,D.W.(2010).Avoiding twisted pixels:ethical guidelines for the appropriate u and manipulation of scientific digital images.Sci.Eng.Ethics 16:639–667.
International Society of Managing and Technical Editors.(2013).Image manipulation in scientific publishing:An interview with Liz Williams,PhD.www.ismte/Interview_with_Liz_Williams-Image_Manipulation_in_Scientific_Publishing_Interview_with_Liz_Williams_PhD.Accesd August 16,2013.
浙江海宁市North,A.J.(2006).Seeing is believing?A beginners’guide to practical pitfalls in image acquisition.J.Cell Biol.172:9–18.
Rossner,M.,and Yamada,K.M.(2004).What’s in a picture?The temptation of image manipulation.J.Cell Biol.166:
11–15.
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Figure 1.Examples of Inappropriate Image Manipulation.
(A)The gel has been cleaned up to hide a stronger band above the main band at 80kD in the rightmost lane.Adjusting the exposure and gamma correction in the magnified view (top right)highlights a pattern of pixel “smearing,”indicated by the red arrow,that differs from the pixel pattern elwhere in the gel image.
(B)Green fluorescent protein expression in the protoplasts appears roughly equivalent with little signal detectable in the control (left).Adjusting the exposure and contrast to the maximum across the image t (bottom),however,demonstrates that the images have not been procesd identically.The first image is completely black,and the color balance between the cond and third clearly differs when the backgrounds are compared.
3148The Plant Cell
DOI 10.1105/tpc.113.250980
; originally published online September 3, 2013;
2013;25;3147-3148Plant Cell Cathie Martin and Mike Blatt
Manipulation and Misconduct in the Handling of Image Data
This information is current as of November 17, 2013
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