小清新女生Frozen-stored granulocytes can be ud for an immunofluorescence test to detect granulocyte
antibodies吴继光
期刊名称： Transfusion
天工开物的作者是谁作者： Motoko Nishimura,Shigeki Mitsunaga,Takeo Juji
怎么办英文年份： 2010年
期号： 第10期
关键词： FBS = fetal bovine rum;Fcγ receptor IIIb = FcγRIIIb;FCM = flow
cytometry;GAH = goat anti‐human;GAM = goat anti‐mou;GAT = granulocyte agglutination test;GIFT = granulocyte immunofluorescence test;LN2 = liquid nitrogen;MAIGA = monoclonal antibody‐immobilized granulocyte assay;MPHA = mixed passive hemagglutination assay
问题性皮肤摘要：BACKGROUND: Donor- and/or recipient-derived granulocyte antibodies are considered to be the main cau of transfusion-related acute lung injury (TRALI), neutropenia, and febrile transfusion re老年人定义
actions. Several types of tests are performed to detect antibodies in donated blood and/or the rum of a transfusion recipient. Becau granulocytes cannot endure the freezing-thawing process, they cannot be stored in liquid nitrogen (LN 2 ). Therefore, testing is time-consuming, becau freshly prepared granulocytes are needed for each testing. An attempt has been made to develop a method that us granulocytes stored in LN 2 for the granulocyte immunofluorescence test (GIFT). STUDY DESIGN AND METHODS: Freshly prepared granulocytes were suspended in a solution of 90-percent fetal bovine rum (FBS) plus 10-percent DMSO and then frozen
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