High resolution protein localization using soft X‐ray
microscopy
期刊名称： Journal of Microscopy
作者： W. Meyer‐Il,D. Hamamoto,A. Nair,S. A. Lelièvre,G. Denbeaux,L.
Johnson,A. L. Pearson,D. Yager,M. A. Legros,C. A. Larabell
五年级上册寒假作业答案>虚弱的反义词是什么什么灯不会亮年份： 2001年
娇艳的女人关键词： Immunogold;protein localization;soft X‐ray microscopy
临危不惧摘要：Soft X‐ray microscopes can be ud to examine whole, hydrated cells up
手镯的含义
嘈杂的意思to 10 µm thick and produce images approaching 30 nm resolution. Since cells are imaged in the X‐ray transmissive 'water window', where organic material absorbs approximately an order of magnitude more strongly than water, chemical contrast enhancement agents are not required to view the distributi
保研流程on of cellular structures. Although living specimens cannot be examined, cells can be rapidly frozen at a preci moment in time and examined in a cryostage, revealing information that
most cloly approximates that in live cells. In this study, we ud a transmission X‐ray microscope at photon energies just below the oxygen edge (λ = 2.4 nm) to examine rapidly frozen mou 3T3 cells and obtained excellent cellular morphology at better than 50 nm
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