FAT16
A lily pollen ASR protein localizes to both cytoplasm and nuclei requiring a nuclear localization signal 期刊名称： Physiologia Plantarum
色大爷操>酒吧ds作者： Huei-Jing Wang,Chia-Mei Hsu,Guang Yuh Jauh,Co-Shine Wang
在西周时期年份： 2005年
期号： 第3期
关键词： cytoplasm;localization;nuclei;plant proteins;pollen
摘要：LLA23, an abscisic acid-, stress- and ripening-induced (ASR) protein, was isolated previously from lily ( Lilium longiflorum ) pollen. Clo examination of the怎么驱赶蝙蝠
软骨板C-terminus of this ASR protein revealed the prence of basic regions reminiscent
春如线of a nuclear localization signal (NLS). Fluorescence microscopy studies using green fluorescent protein (GFP) fusion proteins indicated that the bipartite NLS in LLA23 exhibited nuclear localization properties. Accordingly, mutations in the NLS motifs
of LLA23 defined two regions, either of which was necessary for partial nuclear targeting and both of which were required for complete nuclear localization. In addition, oligonucleotide-directed mutagenesis identified lysine residues within the NLS necessary for nuclear localization. Immunogold localization confirmed that the protein was located
微波炉茄子内容由中国教育图书进出口有限公司引进
