硕士学位论文
BMP4对小鼠诱导性多能干细胞的生物学作用
的初步研究
裘 今
卢进勇
指导教师 谭颖徽 教 授
导师组成员 张纲 副教授 裘松波 副教授 王建华 副教授
开办公司培养单位
陆军军医大学第二附属医院口腔科
申请学位类别 硕士学术学位 专业名称口腔临床医学 论文提交日期
牛肉粿条汤2018年5月
论文答辩日期
2018年5月
答辩委员会主席 田卫东 教授
评 阅 人
陈发明 教授 刘婷姣 教授
二〇
一八年五月
分类号R78 密 级 公 开学 号
2002015211
学校代码
91012
目录
缩略语表 (1)
英文摘要 (2)
中文摘要 (4)
论文正文BMP4对小鼠诱导性多能干细胞的生物学作用的初步研究 (5)
第一章前言 (5)
第二章过表达BMP4对小鼠诱导性多能干细胞牙向分化的影响 (8)
2.1 材料与方法 (8)
2.2 结果 (12)
2.3 讨论 (18)
2.4 小结 (20)
全文总结 (21)
参考文献 (22)
文献综述一诱导多能干细胞向牙源性细胞分化研究 (25)
参考文献 (34)
文献综述二老年人牙缺失的影响及研究现状 (40)
参考文献 (44)
攻读硕士期间发表的文章 (46)
致谢 (47)
大班故事教案缩略语表
英文缩写英文全称中文全称
AMBN Ameloblastin成釉细胞蛋白
ALP alkaline phosphata碱性磷酸酶
BSP Bone sialoprotein骨唾液酸蛋白
BMP4bone morphogenetic protein- 4骨形态发生蛋白-4
西安半坡遗址
CK14Cytokeratin14角细胞蛋白14
CCK8Cell Counting Kit-8细胞计数试剂盒-8 DSPP Dentin sialophosphoprotein牙本质唾液酸焦磷酸蛋白EB embryoid body胚体
iPSCs induced pluripotent stem cells诱导多能干细胞
舒筋健腰丸说明书LIF leukemia inhibitory factor白细胞抑制因子
MEF mou embryonic fibroblasts小鼠胚胎成纤维细胞MSCs menchyma stem cells间充质干细胞
RTFQ PCR realtime fluorescence quantitative PCR荧光定量PCR
TGF transforming growth factor转化生长因子
到家的英文The study of the biological role and theunderlying mechanism of BMP4 onmou induced
pluripotent stem cells
Abstract
Induced pluripotent stem cells (iPSCs) are a unique tool in the field of revolutionary regenerative medicine. It can be the treatment direction of a particular dia. IPS cells can be lf - renewing and can differentiate into many types of cells，thus becoming a potential source of infinite cells in the body. Therefore，the main direction of this rearch is whether iPS cells can provide cell sources for the treatment of oral dias. BMP4 plays an important role in bone formation and development，especially in the bone formation and development of the mouth and limbs. During the development of human embryo， BMP4 is an important signal molecule in the early stage of embryo differentiation. The formation of tooth depends on the expression of BMP4，so as to make tooth germ develop into incisors. In this project，we will explore the methods to solve oral dias by obrving the relationship between the expression of BMP4 and the biological activity of induced pluripotent stem cells in mice.To construct the expression of BMP4 (bone morphogenetic protein- 4，BMP4) lentiviral vector gene and explore its expression of mou induced pluripotent stem cells (iPSCs) effect of biological activity. Methods iPS cell lines stably overexpressing BMP4 were constructed by lentivirus transfection. The activity of cell proliferation was detected by CCK8，and the expression of AMBN，CK14，DSPP，BSP and Runx2 mRNA was detected by Q-PCR. ALP activity was ud to detect the degree of cell differentiation. Results The results of CCK8 showed that the proliferation activity of overexpresd BMP4 could significantly increa the proliferation acti高中军训
vity and ALP activity (P < 0.001) compared with empty vector group. Q-PCR showed that the expression of BMP4 and empty vector group，incread the cell activity of ALP (P < 0.001)，Q-PCR showed that the expression of BMP4 in group Runx2 mRNA expression level was significantly incread (P < 0.001). In addition，the relative expression level of AMBNmRNA in the over expression BMP4 group was 4.473 + 0.456，which was 1.435 times higher than that in the empty carrier group (P < 0.001). Over expression of BMP4 group CK14mRNA expression vector group is 1.79 times (P < 0.001); the relative expression
