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NEB 同尾酶表

更新时间:2023-05-16 22:00:00 阅读： 评论：0

连接互相匹配的粘性末端，产生新的酶切位点
通常连接互相匹配的粘性末端可以产生新的酶切位点。以下组合是通过计算机程序计算出来的，尽管我们希望能尽量保证其准确性，但有些未经实验证实，因此不能保证100%的可行性。如果有同裂酶存在，则只列出其中的一个酶。本表中列出的内切酶均为已商品化的内切酶。
识别序列不够严谨的内切酶（例如，识别多个序列的酶）只在括号内标出相关的序列，但注意这些酶不只切割该序列。
"-"表示连接产物不能被再切割。
时尚英文名灭鼠记狗狗感冒
酶
连接到
可进行切割的酶
Acc65 I
(G/GTACC)
Ban I (G/GTACC)
Acc65 I, Ban I, Kpn I, Nla IV, Rsa I 
BsiW I, BsrG I
Rsa I
Acc I
(GT/CGAC)
Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I
--
(GT/CGAC)
Cla I, BstB I, Taq I
Taq I
Aci I
(C/CGC)
Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I
--
BsaH I (GR/CGCC), HinP1 I, Nar I
Aci I
Hpa II
Hpa II
Acl I
(AA/CGTT)
Acc I (GT/CGAC), Aci I, Cla I, BstB I, HinP1 I, Hpa II, Nar I, Taq I
--
Afl III
(A/CGCGT)
得返利Asc I, BssH II
BstU I, Hha I
(A/CATGT)
BspH I, Nco I, Pci I
Nla III
(A/CGCGT)
Mlu I
Afl III, BstU I, Mlu I
Age I
(A/CCGGT)
Ava I (C/CCGGG), Xma I
Hpa II, Nci I, ScrF I
BsaW I, BspE I
BsaW I, Hpa II
BsrF I (A/CCGGT), SgrA I (CA/CCGGTG)
Age I, BsaW I, BsrF I, Hpa II
NgoM IV
BsrF I, Hpa II
Apa I
(GGGCC/C)
Ban II (GGGCC/C), Bsp1286 I (GGGCC/C)
Apa I, Ban II, Bsp120 I, Bsp1286 I, Hae III, Nla IV, Sau96 I
ApaL I
(G/TGCAC)
Sfc I (C/TGCAG)
Bsg I
Apo I
(A/AATTY)
EcoR I
Apo I, Tsp509 I
(G/AATTY)
EcoR I
Apo I, EcoR I, Tsp509 I
(R/AATTY)
Mfe I, Tsp509 I
Tsp509 I
Asc I混棉是什么料子
(GG/CGCGCC)
Afl III (A/CGCGT), Mlu I
BstU I, Hha I
BssH II
BssH II, BstU I, Cac8 I, Hha I
A I
(AT/TAAT)
Bfa I, Csp6 I, Nde I
--
M I
M I
AsiS I
(GCGAT/CGC)
BsiE I (CGAT/CG)
Dpn II, Pvu I
Pac I
M I
Pvu I
Dpn II, Pvu I
Ava I
(C/CCGGG)
Age I, BsaW I, BspE I, BsrF I (R/CCGGY), NgoM IV, SgrA I (CR/CCGGYG)
Hpa II, Nci I, ScrF I
(C/TCGAG)
Xho I
Ava I, Taq I, Xho I
(C/TCGAG)
Sal I
Taq I
(C/CCGGG)
Xma I
Ava I, BsaJ I, Hpa II, Nci I, ScrF I, Sma I
Ava II
(G/GWCC)
PpuM I (RG/GACCY)
Ava II, Nla IV, Sau96 I
Rsr II
Ava II, Sau96 I
PpuM I (RG/GTCCY)
Ava II, BsmF I, Nla IV, Sau96 I
Avr II
(C/CTAGG)
Nhe I, Spe I, Xba I
Bfa I
Sty I (C/CTAGG)
Avr II, Bfa I, BsaJ I, Sty I
BamH I
(G/GATCC)
Bcl I, Dpn II
Alw I, Dpn II
Bgl II, BstY I (R/GATCY)
Alw I, BstY I, Dpn II
BstY I (G/GATCC)
Alw I, BamH I, BstY I, Dpn II, Nla IV
Ban I
(G/GTACC)
Acc65 I
Acc65 I, Ban I, Kpn I, Nla IV, Rsa I
(G/GCGCC)
Kas I
Ban I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IV
(G/GTACC)
BsiW I, BsrG I
Rsa I
Ban II
(GGGCC/C)
Apa I, Bsp1286 I (GGGCC/C)
Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I
(GAGCT/C)
Bsp1286 I (GAGCT/C), Sac I
Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I
Bcl I
(T/GATCA)
BamH I, BstY I (R/GATCY)
Alw I, Dpn II
Bgl II, Mbo II
Dpn II
Bfa I
(C/TAG)
A I, Csp6 I, M I, Nde I
--
Bgl II
(A/GATCT)
BamH I, BstY I (R/GATCY)
Alw I, BstY I, Dpn II
Bcl I, Dpn II
Dpn II
BsaH I
(GR/CGYC)
Acc I (GT/CGAC), Cla I, BstB I, Taq I
--
(GA/CGYC)
Aci I, HinP1 I
Hga I
(GG/CGYC)
Aci I, HinP1 I
Hha I
(GG/CGYC)
Hpa II
Aci I
(GA/CGYC)
Nar I
眼睛红怎么回事BsaH I, Hga I
(GG/CGYC)
Nar I
Ban I, BsaH I, Hae II, Hha I, Nar I, Nla IV
BsaW I
(W/CCGGW)
Age I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)
Age I, BsaW I, BsrF I, Hpa II
Ava I (C/CCGGG), Xma I
Hpa II, Nci I, ScrF I
BspE I
BsaW I, BspE I, Hpa II
BsrF I (R/CCGGY), NgoM IV
BsrF I, Hpa II
NgoM IV
Hpa II
BsiE I
(CGAT/CG)
Pac I
M I
(CGAT/CG)
Pvu I
BsiE I, Dpn II, Pvu I
(CGGC/CG)
Sac II
Aci I
BsiHKA I
(GTGCA/C)
Bsp1286 I (GTGCA/C)
BsiHKA I, Bsp1286 I
Bsp1286 I (GAGCA/C)
BsiHKA I, Bsp1286 I
Bsp1286 I (GAGCT/C), Sac I
Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I
Nsi I
--
Pst I, Sbf I
Bsg I
BsiW I
(C/GTACG)
Acc65 I, Ban I (G/GTACC), BsrG I
Rsa I
Bsp1286 I
(GGGCC/C)
Apa I, Ban II (GGGCC/C)
Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I
(GTGCA/C)
BsiHKA I
ApaL I, BsiHKA I, Bsp1286 I
(GGGCC/C)
Ban II (GGGCC/C)
Ban II, Bsp1286 I
(GAGCT/C)
Ban II (GAGCT/C), BsiHKA I, Sac I
Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I
(GWGCW/C)
BsiHKA I
BsiHKA I, Bsp1286 I
(GTGCA/C)
Nsi I
--
(GTGCA/C)
Pst I, Sbf I
Bsg I
BspE I
(T/CCGGA)
Age I, BsaW I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)
BsaW I, Hpa II
Ava I (C/CCGGG), Xma I
Hpa II, Nci I, ScrF I
BsaW I
BsaW I, BspE I, Hpa II
BsrF I (R/CCGGY), NgoM IV
Hpa II
BspH I
(T/CATGA)
Afl III (A/CATGT), Nco I, Sty I (C/CATGG)
Nla III
BsrF I
(A/CCGGY)
Age I, BsaW I
Age I, BsaW I, BsrF I, Hpa II
(G/CCGGY)
Age I, BsaW I, NgoM IV
BsrF I, Hpa II
(R/CCGGY)
Ava I (C/CCGGG), Xma I
Hpa II, Nci I, ScrF I
(A/CCGGY)
BsaW I, BspE I
BsaW I, Hpa II
(R/CCGGY)
BsaW I, BspE I
Hpa II
(G/CCGGY)
NgoM IV
BsrF I, Cac8 I, Hpa II, Nae I
(CR/CCGGYG)
SgrA I
BsrF I, Hpa II
BsrG I
(T/GTACA)
Acc65 I, Ban I (G/GTACC), BsiW I
Rsa I
BssH II
(G/CGCGC)
Afl III (A/CGCGT), Mlu I
BstU I, Hha I
Asc I
BssH II, BstU I, Cac8 I, Hha I
BstB I
(TT/CGAA)
Acc I (GT/CGAC), Cla I, Taq I
Taq I
Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I
--
BstY I
(A/GATCY)
BamH I, Bgl II
Alw I, BstY I, Dpn II
(G/GATCY)
BamH I
Alw I, BamH I, BstY I, Dpn II, Nla IV
(R/GATCY)
Bcl I, Dpn II
Dpn II
(G/GATCY)
Bcl I, Dpn II
Alw I, Dpn II
(A/GATCY)
Bgl II
Bgl II, BstY I, Dpn II
Cla I
(AT/CGAT)
Acc I (GT/CGAC), BstB I, Taq I
Taq I
Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I
--
Dpn II/Mbo I/
Sau3A I (/GATC)
BamH I, BstY I (R/GATCC)
Alw I, Dpn II
Bcl I, Bgl II, BstY I (R/GATCY)
Dpn II
Eae I
(Y/GGCCR)
PspOM I
Hae III, Sau96 I
(C/GGCCR)
Eag I
BsiE I, Eae I, Eag I, Hae III
(T/GGCCR)
Eag I
Eae I, Hae III
(C/GGCCR)
Not I
Aci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae III
(T/GGCCR)
Not I
Aci I, Eae I, Fnu4H I, Hae III
Eag I
(C/GGCCG)
PspOM I
Hae III, Sau96 I
Eae I (Y/GGCCR)
Eae I, Hae III
Eae I (C/GGCCG)
BsiE I, Eae I, Eag I, Hae III
Not I
Aci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae III
EcoR I
(G/AATTC)
Apo I (G/AATTC)
Apo I, EcoR I, Tsp509 I
Apo I (R/AATTY)
Apo I, Tsp509 I
Mfe I, Tsp509 I
Tsp509 I
HinP1 I
(G/CGC)
Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I
--
Aci I, BsaH I (GR/CGCC), Nar I
Hha I
BsaH I (GR/CGTC)
Hga I
Hpa II
Aci I
Hpa II/Msp I
(C/CGG)
Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I
--
Aci I, BsaH I (GR/CGCC), HinP1 I, Nar I
Aci I
Kas I
(G/GCGCC)
Ban I (G/GCGCC)
Ban I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IV
Mfe I
(C/AATTG)
Apo I (R/ATTTY), EcoR I, Tsp509 I
Tsp509 I
Mlu I
(A/CGCGT)酿茄子
Afl III (A/CGCGT)
Afl III, BstU I, Mlu I
Asc I, BssH II
BstU I, Hha I
M I
(T/TAA)
A I
M I
Bfa I, Csp6 I, Nde I
--
Nar I
(GG/CGCC)
Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I
--
Aci I, HinP1 I
Hha I
BsaH I (GR/CGCC)
Ban I, BsaH I, Hae II, Hha I, Nar I, Nla IV
BsaH I (GR/CGTC)
BsaH I, Hga I
Hpa II
Aci I
Nco I
(C/CATGG)
Afl III (A/CATGT), BspH I
Nla III
Sty I (C/CATGG)
BsaJ I, Nco I, Nla III, Sty I
Pci I
Nla III
Nde I
(CA/TATG)
A I, Bfa I, Csp6 I, M I
--
NgoM IV
(G/CCGGC)
Age I, BsaW I, BsrF I (R/CCGGY), SgrA I
BsrF I, Hpa II
Ava I (C/CCGGG), Xma I
Hpa II, Nci I, ScrF I
BsaW I, BspE I
Hpa II
BsrF I (R/CCGGC), SgrA I
BsrF I, Cac8 I, Hpa II, Nae I咏兔
Nhe I
(G/CTAGC)
Avr II, Spe I, Sty I (C/CTAGG), Xba I
Bfa I
Nla III
(CATG/)
Sph I, Nsp I
Nla III
Not I
(GC/GGCCGC)
PspOM I
Aci I, Eae I, Fnu4H I, Hae III
Eag I
Aci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae III
Eae I (Y/GGCCR)
Aci I, BsiE I, Eae I, Fnu4H I, Hae III
Nsi I
(ATGCA/T)
BsiHKA I (GTGCA/C), Bsp1286 I (GTGCA/C), Pst I, Sbf I
--
Nsp I
(RCATG/Y)
Nla III, Sph I
Nla III
Pac I
(TTAAT/TAA)
BsiE I (CGAT/CG), Pvu I
M I
Pci I
(A/CATGT)
Afl II (A/CATGT), BspH I, Nco I, Sty I (C/CTAGG)
Nla III
PpuM I
(RG/GWCCY)
Ava II, Rsr II
Ava II, Sau96 I
(GG/GTCCY)
Ava II, Rsr II
Ava II, BsmF I, Nla IV, Sau96 I
(GG/GACCY)
Ava II, Rsr II
Ava II, Nla IV, Sau96 I
PspOM I
(G/GGCCC)
Eae I (Y/GGCCR), Eag I
Hae III, Sau96 I
Not I
Aci I, Fnu4H I, Hae III, Sau96 I
Pst I
(CTGCA/G)
BsiHKA I, Bsp1286 I (GTGCA/C)
Bsg I
Nsi I
--
Sbf I
Pst I
Pvu I
(CGAT/CG)
AsiS I
Dpn I, Pvu I
Pac I
M I
BsiE I (CGAT/CG)
BsiE I, Dpn II, Pvu I
Rsr II
(CG/GWCCG)
Ava II, PpuM I (RG/GACCY)
Ava II, Sau96 I
PpuM I (RG/GACCY)
Ava II, Nla IV, Sau96 I
PpuM I (RG/GTCCY)
Ava II, BsmF I, Nla IV, Sau96 I
Sac I
(GAGCT/C)
Ban II (GAGCT/C), BsiHKA I, Bsp1286 I (GAGCT/C)
Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I
Sac II
(CCGC/GG)
BsiE I (CGGC/CG)
Aci I
Sal I
(G/TCGAC)
Ava I (C/TCGAG), Xho I
Taq I
Sbf I
(CCTGCA/GG)
BsiHKA I, Bsp1286 I (GTGCA/C)
Bsg I
Nsi I
--
Pst I
Pst I
Sfc I
(C/TGCAG)
ApaL I
Bsg I
SgrA I
(CR/CCGGYG)
See BsrF I
 
Spe I
(A/CTAGT)
Avr II, Nhe I, Sty I (C/CTAGG), Xba I
Bfa I
Sph I
(GCATG/C)
Nla III, Nsp I
Nla III
Sty I
Avr II
Avr II, Bfa I, BsaJ I, Sty I
(C/CTAGG)
Nhe I, Spe I, Xba I
Bfa I
(C/CATGG)
BspH I
Nla III
Nco I
BsaJ I, Nco I, Nla III, Sty I
Taq I
(T/CGA)
Acc I (GT/CGAC), Cla I, BstB I
Taq I
Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I,Hpa II, Nar I
--
Tsp509 I
(/AATT)
Apo I (R/AATTY), EcoR I, Mfe I
Tsp509 I
Xba I
(T/CTAGA)
Avr II, Nhe I, Spe I, Sty I (C/CTAGG)
Bfa I
Xho I/Tli I
(C/TCGAG
Ava I (C/TCGAG)
Ava I, Taq I, Xho I
Sal I
Taq I
Xma I
(C/CCGGG
Age I, BsaW I, BspE I, BsrF I, NgoM IV, SgrA I
Hpa II, Nci I, ScrF I
Ava I (C/CCGGG)
Ava I, BsaJ I, Hpa II, Nci I, ScrF I, Sma I, Xma I

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