pcDNA3.1(+)
pcDNA3.1(-)
Catalog nos. V790-20 and V795-20, respectively
Version I
081401
28-0104
tech_
ii
Table of Contents
Table of Contents ......................................................................................................... iii Important Information ................................................................................................... v Purchar Notification ................................................................................................. vi Methods .........................................................................................................................1
Overview...................................................................................................................................................................1
Cloning into pcDNA3.1 ............................................................................................................................................2
Transfection ..............................................................................................................................................................6
Creation of Stable Cell Lines ....................................................................................................................................7
Appendix ...................................................................................................................... 10 pcDNA3.1 Vectors .................................................................................................................................................10 pcDNA3.1/CAT ......................................................................................................................................................12
Technical Service....................................................................................................................................................13
References...............................................................................................................................................................15
iii
