Journal of Integrative Agriculture 2018, 17(8): 1843–1851
RESEARCH ARTICLE
Available online at
ScienceDirect
iTRAQ-bad quantitative proteomic analysis reveals key pathways responsible for scurs in sheep (Ovis aries)
HE Xiao-hong1, 2*, CHEN Xiao-fei1, 2, 3*, PU Ya-bin1, 2, GUAN Wei-jun1, 2, SONG Shen1, 2, ZHAO Qian-jun1, 2, LI Xiang-chen1, 2, JIANG Lin1, 2, MA Yue-hui1, 2
1 Institwps云
u家具品牌排名
te of Animal Science, Chine Academy of Agricultural Sciences, Beijing 100193, P.R.China
2个人履历范本
CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chine Academy of Agricultural Sciences, Beijing 100193, P.R.China
3 Institute of Animal Science, Enshi Autonomous Prefecture Academy of Agricultural Sciences, Enshi 445000, P.R.China
Abstract
Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant association to the polled locus, relaxin-like receptor 2 (RXFP2). However, the molecular mechanism underlying the development of scurs remains largely unknown. In the prent study, we performed an iTRAQ-bad quantitative proteomic analysis of horn tissues from both scurs and normal two-horned and four-horned individuals among Altay sheep to identify th
e differentially expresd proteins (DEPs) responsible for the scurs phenotype. In total, 232 proteins showed significant differential expression, and the most significant Gene ontology categories were the adhesion process (biological adhesion (P=4.0710–17) and cell adhesion (P=3.710–16)), multicellular organismal process (single-multicellular organism process (P=2.0610–11) and multicellular organismal process (P=2.2910–11)) and extracellular process (extrac八仙过海的神话故事
ellular matrix organization (何以家为
P=4.7710–16) and extracellular structure organization (P工程性质
=4.9310–16)). Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that extracellular matrix (ECM)-receptor interactions and focal adhesion pathways were the most significant pathways. This finding is consistent with the reduced formation of extracellular matrix in scurs and the development of deformed horn tissues. Our study helps to elucidate the inheritance pattern of sheep horn traits from t班风学风
he perspectives of downstream expresd proteins.
Keywords: scurs, sheep, iTRAQ, horn development, ECM-receptor interaction pathway
1. Introduction
Horns are cloly related to natural and x lection
(Johnston et al. 2013), and also exhibit a variety of
phenotypes, including normal two horns, scurs (deformed
horns) (Ibn 1944), polled (abnce of horns) (Ryder 1983)
and multi-horns (more than two horns) (Elwes 1913; Ritchie
1913; Blunn 1943; Maiwashe and Blackburn 2004). White
and Ibn (1936) originally propod a horn inheritance Received 24 October, 2017 Accepted 29 January, 2018
HE Xiao-hong, E-mail: ; Correspondence
JIANG Lin, E-mail: ; MA Yue-hui, Tel/Fax: +86-
10-62813463, E-mail: yuehui.
* The authors contributed equally to this study.
2018 CAAS. Publishing rvices by Elvier B.V. All rights
rerved.
doi: 10.1016/S2095-3119(17)61894-X
