Aerobic Bacteria Count = 152
马拉松跑全程是多少A red indicator dye in the plate colors the colonies.Count all red colonies regardless of their size or color intensity.
3Petrifilm
™
Aerobic Count Plate
This guide familiarizes you with results on 3M ™Petrifilm ™Aerobic Count Plates. For more information, contact the official 3M Microbiology Products reprentative nearest you.
Interpretation Guide二十四节气手抄报
1
The Petrifilm Aerobic Count (AC) plate is a ready-made culture medium system that contains Standard Methods nutrients, a cold-water-soluble gelling agent, and an indicator that facilitates colony enumeration. Petrifilm AC plates are ud for the enumeration of aerobic bacteria.
Count = 0
It is easy to interpret the Petrifilm AC plate. Figure 2shows a Petrifilm AC plate without colonies.
Count = 16
Figure 3 shows a Petrifilm AC plate with a few bacterial colonies.
3M ™
Petrifilm
™
Aerobic Count Plate
3
2
Estimated Count = 560
When colonies number more than 250, as in figure 5,estimate the count. Determine the average number of colonies in one square (1 cm 2) and multiply it by 20 to obtain the total count per plate. The inoculated area on a Petrifilm AC plate is approximately 20 cm 2.
Count = 143
The preferable counting range on a Petrifilm AC plate is 25–250 colonies. See figure 4.
4
5
Count = TNTC (Estimated count = 103) Figure 6 shows a Petrifilm AC plate with colonies that are too numerous to count (TNTC).
Count = TNTC (Estimated count = 103) Occasionally, distribution of colonies appears uneven,
as shown in figure 8. This is also an indication
of a TNTC result. Count = TNTC (Estimated count = 107) The colonies on the Petrifilm AC plate in fi
gure 9appear countable at first glance. However, when you look cloly at the edge of the growth area, you can e a high concentration of colonies. Record this as a TNTC result. Count = TNTC (Estimated count = 105) With very high counts, the entire growth area may turn pink, as shown in figure 7. You might obrve individual colonies only at the edge of the growth area. Record this as a TNTC result.
TNTC(Too Numerous to Count) To obtain a more accurate count, dilute the sample further
67
89
Gel Liquefication and Food Particles
Estimated Count = 160
A few species of bacteria liquify the gel in the
Petrifilm AC plate, as shown in figure 10. When
this occurs, determine the average count in a few
禁养区unaffected squares and then multiply it by 20 to
obtain the estimated count. Do not count red spots李恩珠红字
within the liquified area.
10清泉石上流的上一句
Count = 83只见树木
Becau colonies on Petrifilm AC plates are red, you
can distinguish them from opaque, irregularly shaped
food particles (e circles 1 and 2).
1
2
11
Prepare a 1:10 or greater dilution of food sample. Weigh or pipette food product into an appropriate container such as a stomacher bag, dilution bottle, Whirl-Pak ®bag, or other sterile container.
(≤46°F). U before expiration date on package. In areas of high humidity where condensate may be an issue, it is best to allow packages to reach room temperature before opening.
制定安全生产规章制度的原则over and tape shut.
(≤77°F) and ≤50%RH.Do not refrigerate opened packages.U Petrifilm plates within one month after opening.
onto center of bottom film.计算机组装与维护
Sample Preparation
4
Continued - over
0.0425 g/L of KH 2PO 4adjusted to pH 7.2), 0.1% peptone water, peptone salt diluent (ISO method
6887), buffered peptone water (ISO method 6579),saline solution (0.85 - 0.90%), bisulfate-free letheen broth, or distilled water. Adjust pH of the diluted sample between 6.6 and 7.2 :
• for acid products, u 1N NaOH,• for alkaline products, u 1N HCI.
