龙女奶荒
Characterization of site-specific N-glycopeptide isoforms of alpha-1-acid glycoprotein from an interlaboratory study using LC-MS/MS
华为系统更新
珍珠圆子的家常做法期刊名称： Journal of Proteome Rearch
交通劝导
作者： Lee, Ju Yeon,Lee, Hyun Kyoung,Park, Gun Wook,Hwang, Heeyoun,Jeong, Hoi Keun,Yun, Ki Na,Ji, Eun Sun,Kim, Kwang Hoe,Kim, Jun Seok,Kim, Jong Won,Yun, Sung Ho,Choi, Chi-Won,Kim, Seung Il,Lim, Jong-Sun,Jeong, Seul-Ki,Paik, Young-Ki,Lee, Soo-Youn,Park, Jisook,Kim, Su Yeon,Choi, Young-Jin,Kim, Yong-In,Seo,
Jawon,Cho, Je-Yoel,Oh, Myoung Jin,Seo, Nari,An, Hyun Joo,Kim, Jin Young,Yoo, Jong Shin
清淡的食物有哪些年份： 2016年
黑人明星
关键词： PTM (post translational modification);isoforms;site-specific N-
glycopeptide;interlaboratory study
摘要：Glycoprotein conformations are complex and heterogeneous. Currently, site-specific characterization of glycopeptides is a challenge. We sought to establish an efficient method of N-glyco
200字加油稿protein characterization using mass spectrometry (MS). Using alpha-1-acid glycoprotein (AGP) as a model N-glycoprotein, we identified its tryptic N-glycopeptides and examined the data reproducibility in ven laboratories running different LC-MS/MS platforms. We ud three test samples and one blind sample to evaluate instrument performance with entire sample preparation workflow. 165 site-specific N-glycopeptides reprentative of all N-glycosylation sites were identified from AGP 1 and AGP 2 isoforms. The glycopeptide fragmentations by collision-induced dissociation or higher-energy collisional dissociation (HCD) varied bad on the
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