Purification and characterization of an erythromycin estera from an erythromycin-resistant
致富带头人Pudomonas sp
大竹东湖公园>黄花鱼的制作方法期刊名称: FEMS Microbiology Letters
作者: Yong-Hak Kim,Chang-Jun Cha,Carl E. Cerniglia
年份: 2002年
老公过生日
回故乡期号: 第 2期
重视用英语怎么说关键词: Aquaculture;Erythromycin A;Erythromycin A enol旗杆的读音
ether;Oleandomycin;Mercuric chloride
摘要:An erythromycin estera (molecular mass 51200 Da) was purified from Pudomonas sp. GD100, which was isolated from a salmon hatchery diment
sample from Washington State. The p I of the protein was 4.5–4.8. The enzyme was inhibited by 1 mM mercuric acid, and had the substrate specificity for structurally related 14-membered macrolides, which decread in the order of oleandomycin, erythromycin A and erythromycin A enol ether. The activity for erythromycin A
varied with temperature, but the effect of pH was minimal at pH 6.0–9.0. The half-life of the enzyme was estimated to be 8.9 h at 35°C and 0.23 h at 55°C, and the
深字开头的成语activation energy of the catalytic reaction of erythromycin A was estimated at 16.2 kJ mol 1 .
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