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Cloning and characterization of a novel cold-active endoglucana establishing a new subfamily of glycosyl hydrola family 5 from a psychrophilic
单身的句子deep-a bacterium
期刊名称: FEMS Microbiology Letters哪种大米好吃>硬盘格式化后能恢复数据吗
作者: Yang Jinying,Dang Hongyue
耳机煲机教程>关于过生日的作文期号: 第1期
关键词: deep a;\n Pudomonas\n ;cold-active;cellula;psychrophile
嫁与春风不用媒摘要:The gene of a novel endo--1,4-glucana (named Cel5M) was isolated
from the psychrophilic deep-a bacteria Pudomonas sp. MM15. The deduced protein quence lacked the typical cellula domain structures of the carbohydrate-binding module and the linker region. Cel5M showed relatively higher activity toward carboxymethyl cellulo, but much lower activity
toward p-nitrophenyl--d-galactopyranoside and no activity toward avicel. Cel5M was identified as a cold-active cellula with an optimal temperature of 30 °C and it was active within a narrow pH range with an optimum at pH 4.5. Phylogenetic analysis showed that Cel5M reprented a new subfamily of the glycosyl hydrola family 5, reprenting an opportunity for rearch into and applications of novel cold-active cellulas.作文本
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