Establishment and Characterization of a New Human Cell line (EJ) Derived from Endometrial
Carcinoma
期刊名称： Human Cell
叶绿素a颜色山西历史名人作者： Keiichi Isaka,Hirotaka Nishi,Toshihide Nakada,Yumi Osakabe,Mitsuyasu Hokamura,Hiromi Serizawa,Yoshiro Ebihara,Masaomi Takayama
年份： 2010年春远
南方小年吃什么小的花语关键词： cell line;endometrial carcinoma;telomera activity;estrogen receptor
胆囊炎治疗>淡奶油打发摘要：We prent a new cell line, EJ, established from an invasive endometrioid adenocarcinoma of the uterine corpus in a 56-year-old patient The cells show rapid growth in culture with a doubling time of 16 h and high migration activity. Monolayer-cultured cells were polygonal in shape showing a tendency to pile up without contact inhibition. Subcutaneous transplantation of the EJ cells into nude mice formed solid tumors that were histologically diagnod as adenocarcinoma, whereas no metastas
is was obrved. Cultured EJ cells produced tissue polypeptide antigen (TPA). Genetic and molecular analys revealed high telomera activity but not estrogen receptor a expression. Using the DNA quencing technique, we have screened EJ cells for p53 mutation in exon 5 to 8 but no mutation of p53 was obrved. This cell line appears to 兽医硕士
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