萝卜的做法柚子简笔画Molecular characterization and enzymatic analysis of juvenile hormone epoxide hydrola genes in the red flour beetle Tribolium castaneum 期刊名称： Inct Molecular Biology
始作俑者作者： T. Tsubota,T. Nakakura,T. Shiotsuki
年份： 2010年
新版绝代双骄期号： 第3期
关键词： JHEH;cDNA cloning;epoxide hydrola;detoxification
西游记精彩句段enzyme;Coleoptera
摘要：Juvenile hormone epoxide hydrolas (JHEHs) degrade juvenile hormones (JHs) and are important for JH titre regulation. Here, we report the cloning and analysis of five jheh-related (jheh-r1–r5) genes in the red flour beetle, Tribolium castaneum, a model species for the coleopteran incts. T. castaneum JHEH-r (TcJHEH-r) proteins show high homology to lepidopteran JHEHs and also to human microsomal epoxide hydrola. In the phylogenetic tree, Tcjheh-rs were clustered, and interesti
ngly, they were also clustered in the genome. Examination of enzymatic activities using recombinant TcJHEH-r proteins showed that TcJHEH-r3 had strong degradation activity for JH III, whereas TcJHEH-r4 had weak activity. The study has yielded significant information that will facilitate further analysis of JHEHs and epoxide hydrolas.国际四大会计师事务所
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