专利名称：Strain for the production of 6-
帝王托帕石demethyltetracycline, method for producing
the strain and vector for u in the method 发明人：Michael J. Ryan
申请号：US09/031855
申请日：19980227
公开号：US05965429A
公开日：
文献检索步骤
19991012
专利内容由知识产权出版社提供
摘要：Recombinant S. aureofaciens cells are provided. The cells compri: (a) at least one CTC 11 gene; and (b) optionally
< P>(i) a CTC 09 gene;
祝福老师的句子(ii) a CTC 03 gene; or
(iii) a combination thereof;
wherein:
the CTC 11 gene is chromosomal, extra- chromosomal, or chromosomal and extra-chromosomal;
the CTC 09 gene, CTC 03 gene, or a combination thereof is chromosomal, extra-chromosomal, or a combination thereof; expression of the CTC 11 gene is enhanced over that of a wild- type S. aureofaciens cell; and
optionally, the CTC 09 gene, the CTC 03 gene, or both of the CTC 09 gene and the CTC 03 gene are inactivated.
The prent invention also contemplates vector pLP21329 and vectors for allelic replacement in a S. aureofaciens host cell. The vectors compri:
(a) a functional E. coli origin of replication;
励志小故事50字(b) a functional Streptomyces origin of replication;
(c) a functional gene that imparts a positively lectable phenotype on the host cell; and
((d) a ribosomal S12 gene which is expresd in Streptomyces such that it imparts nsitivity to streptomycin to the host cell. In another embodiment, a method of mutating a target gene of a biosynthetic pathway of Streptomyces is disclod. The method compris
(a) replacing the genomic copy of the target gene with a lectable marker gene through homologous recombination to form a first recombinant strain; and
(b) replacing the lectable marker gene in the first recombinant strain with an altered copy of the target gene through homologous recombination to form a cond recombinant strain.
申请人：AMERICAN CYANAMID COMPANY
党的组织生活制度代理机构：Darby & Darby
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