专利名称:GLUCOSE ISOMERASE MUTANTS, THE USE THEREOF AND THE DNAS ENCODING THE
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发明人:WANG, JUN,JIN, CAIKE,FU,
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RONGZHAO,SHEN, DONG古巷里
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申请号:EP06805105
申请日:20061030积极向上广播稿
公开号:EP1956083A4
公开日:
20090225
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专利内容由知识产权出版社提供
摘要:This invention provides a ries of recombinant gluco isomeras with improved catalytic activity and thermostability obtained by using recombinant techniques. The recombinant gluco isomeras compri amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, rine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and posss a higher catalytic activity than that of the wild-type when using D-gluco as substrate. The recombinant gluco isomeras can be ud for direct production of high fructo corn syrup containing 55.% [wt] or higher concentration of fructo.
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