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Production, purification and characterization of thermophilic lipa from Bacillus sp. THL027期刊名称： FEMS Microbiology Letters几号七夕
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作者： Dharmsthiti Saovanee,Luchai Sudaporn
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年份： 1999年
期号： 第2期
电脑开机时间关键词： Production;Characterization;\n Bacillus sp. lipa;Thermophilic lipa
虚拟充值摘要：A low-cost medium, MGRS, has been developed for growth and lipa production from Bacillus THL027 at 65°C and pH 7.0. MGRS was compod of 2% (v/v) buffer solution (7.3% (w/v) Na2HPO4, 3.2% (w/v) KH2PO4, pH 7.2), 40 g ml1 FeSO4 and 40 g ml1 MgSO4, 0.1% (w/v) (NH4)2SO4 supplemented with 3% NaCl, 0.1% gluco, 1.0% rice bran oil and 0.5% (w/v) rice bran. The lipa was purified 2.6-fold to apparent homogeneity by ultrafiltration and gel filtration chromatography. Its molecular mass was 69 kDa. The purified enzyme was characterized for its general physical properties.
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