PA THOLOGICAL SECTIONING TECHNIQUE
病理切片技术
Quality pathology is the key to making the right pathology. The preparation of a high-quality pathological ction is not completely dependent on advanced instruments or technicians with higher qualifications. It requires technicians to have a strong n of responsibility in daily work, improve quality awareness, and pay attention to each work link. carry out. The pathological specimens of cardiac hospitals are mainly compod of heart valves, blood vesls, myocardium, auricles, myxomas and other tissues. The preparation process of pathological ctions is also different from other types of tissues. The following are some of the experiences and experiences of the author in the pathological ction preparation work in the Cardiac Specialized Hospital for more than 10 years, only for reference by peers.
1 Fixing is sufficient and timely Specimen fixation is the first step in the production of the film. The fixed role is: 1 Keep cells in a similar shape to life, preventing autolysis and corruption;
落井下石的意思2 Keep the special components in the cell similar to tho in the living state;
为何爱会伤人3 to facilitate the differentiation of different organizational components;
什么汤补血>风度翩翩4 is conducive to slicing. The concentration of the fixative solution should not be too high or too low. If the concentration is too high, the surface layer of the specimen will be hardened rapidly to form a hard shell, which will affect the penetration of the fixative into the specimen, resulting in poor internal fixation of the specimen; If the concentration is too low, it will not be fixed, resulting in tissue autolysis and spoilage. The correct fixing method is: After the tissue was isolated, the tissue was first fixed with 4% neutral formaldehyde solution. The amount of fixative should be sufficient (usually
5 to 10 times the volume of the specimen). For larger specimens, they can be cut along their maximum profile and then fixed.
2 According to the specification, the specimen can be taken after it has been fixed. The location of the material should be reasonable, and the tissue block should be reprentative, and the normal or unaffected tissue associated with the lesion should be displayed as much as possible. Take enough materials. The tissue block is about 0.20~0.30 cm thick. For some tissues, the materials
口罩怎么画should be flexibly mastered, and the hard and tough tissues such as valves and blood vesls with vere calcification should be thinned appropriately, and some soft tissues such as auricles and myocardium can be appropriately thickened. When embedded, the fine tissue should be embedded i
n the same plane. When large pieces of tissue are embedded, they should be flattened to prevent incomplete slicing and disordered intra-slice tissue. For the skin, blood vesl wall, and cyst wall tissue, it should be buried to obrve its various levels. Slices should u sharp slicer blades, and new blades should be replaced in time. Slices should be cut intact, cut to the largest surface of the tissue to prevent misd diagnosis. Small specimens should be noted that they cannot be repaired too much. Large specimens should be carefully cut. In order to cut the whole slice and prevent the cracks in the tissue, the hard and crisp part should be placed on the upper end. In the ca of verely calcified tissue, it should be gently cut to prevent the tissue from being hollow due to vibration. In order to maintain the proper hardness of the wax block, ice cubes are often ud in the ctions to cool the tissue pieces. The patch should be appropriate, pay attention to the position of the wax sheet, leave space for labeling, pay attention to neat and beautiful. After picking up the wax, write the number immediately to avoid confusion with the next ca.摄影艺考
法宣在线登录入口3. The dyeing should be bright, and the aling should be careful. The HE dyeing process is strictly performed. The hematoxylin dyeing solution should be prepared well, and the quality of the dyeing solution directly affects the quality of the dyeing. Hydrochloric acid alcohol should be properly differentiated, over-differentiated, and the nucleus color is not clear. Insufficient differentiation and da
憧憬的意思是什么rk coloration make the nucleus pulpy state unclear. The quality of hematoxylin staining of the nucleus is controlled under the microscope if necessary. Eosin staining should be appropriate, not too deep or too light, HE staining should be brightly colored, red and blue distinct, clear contrast. Seal the drops of the alant to the right amount, not too little and not spillover is better, the slides are clean and tidy.
4. Pathologists professional quality and work responsibility Heart pathology production from the organization of fixation, material extraction, dehydration, embedding, slicing, staining and other procedures, requiring technical personnel to strictly implement the technical operating procedures of clinical technical practices, do a good job All aspects of production, and constantly explore, summarize, and innovate in the work, can produce high-quality pathological ctions, providing a strong technical guarantee for pathological diagnosis.
