微生物多样性（扩增子/16S rDNA测序）
分析报告内容解析
——α多样性分析
主要内容
α多样性指数分析内容及意义1
假设英语
α多样性分析在论文中的描述2
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α多样性指数分析内容及意义
a)群落生态学中研究微生物多样性，通过单样品的多样性分析（α[Alpha]多样性）可以反映
微生物群落的丰度和多样性，包括一系列统计学分析指数估计环境群落的物种丰度和多样性。酱油肉
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b)α多样性指分析主要包括：
①菌群丰度（Community richness）指数计算：Chao、Ace指数；收费英语
②菌群多样性（Community diversity）指数计算：Shannon、Simpson指数。
——获得指数计算汇总表格。
c)α多样性可视化分析图（来源：指数分析汇总表）：
稀释性曲线（Rarefraction Curve）；Shannon-Wiener曲线；Rank-Abundance曲线；Specaccum曲线
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α多样性分析在论文中的描述
a)稀释性曲线和Shannon-Wiener曲线结合使用：
例如1：Shannon and Rarefaction analysis showed that,although new phylotypes can be obtained by additional quencing,most of the gut microbial diversity in each sample was captured with the current quencing depth.After rarefying the quencing depth among all the samples using bootstra应的词语
面点p (XX,XXX reads per sample),Shannon diversity index and rarefaction OTU estimates were calculated. There was no significant difference in the richness(as indicated by rarefaction OTU estimates)and diversity between Group A and Group B in this study.
如有显著差异：while Group B significantly reduced both the richness and diversity of the microbiota(P<0.05OR Show in fig.)
a)稀释性曲线和Shannon-Wiener曲线结合使用：
例如2：The richness of oral bacterial communities in three oral habitats of periodontitis patients and the control cohort were estimated by rarefaction and Chao1,and diversity was estimated by Shannon diversity index and Simpson diversity.The shape of the rarefaction curves indicated new phylotypes would be expected with additional quencing.However,the Shannon diversity index curves of all samples reached plateaus with the current quencing,suggesting that most diversity had already been captured.
