Commonly ud strains openwetware/wiki/E._coli_genotypes 1.AG1
endA1 recA1 gyrA96 thi-1 relA1 glnV44 hsdR17(r
K - m
K
+)
2.AB1157
thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0,
glnV44(AS), galK2(Oc), LAM-, Rac-0, hisG4(Oc), rfbC1, mgl-51,
rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thi-1∙Bachmann BJ: Derivation and genotypes of some mutant derivatives of Escherichia coli K-12.
Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology (Edited by: F C Neidhardt J L Ingraham KB Low B Magasanik M Schaechter H E Umbarger). Washington, D.C., American Societ
y for Microbiology 1987, 2:1190-1219.
See CGSC#1157
素质教育观
3.BL21
E. coli B F- dcm ompT hsdS(r
B - m
B
-) gal [malB+]
K-12
(λS)
∙The "malB region" was transduced in from the K-12 strain W3110 to make the strain Mal+λS. See Studier et al. (2009) J. Mol. Biol.
394(4), 653 for a discussion of the extent of the transfer.
∙Stratagene E. coli Genotype Strains
4.BL21(AI)
F– ompT gal dcm lon hsdS
B (r
B
- m
B
-) araB::T7RNAP-tetA
∙an E. coli B strain carrying the T7 RNA polymera gene in the araB locus of the araBAD operon q.
∙Transformed plasmids containing T7 promoter driven expression are represd until L-arabino induction of T7 RNA polymera.
∙Derived from BL21.
∙See the product page for more information.
∙Brian Caliendo (Voigt lab) reported trouble getting the Datnko and Wanner (2000) plasmid pCP20 to transform into this strain, when other strains transformed fine. Cau is unknown.
5.BL21(DE3)
F–ompT gal dcm lon hsdS
B (r
B
-m
B
-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7
nin5])
∙an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymera gene and lacI q
∙Transformed plasmids containing T7 promoter driven expression are represd until IPTG induction of T7 RNA polymera from a lac
promoter.
∙Derived from B834 (Wood, 1966) by transducing to Met+.
∙See the original Studier paper or the summary in Methods in Enzymology for more details.
∙Whole genome quence available [1]
6.BL21 (DE3) pLysS
F- ompT gal dcm lon hsdS
B (r
B
- m
B
-) λ(DE3) pLysS(cm R)
∙pLysS plasmid chloramphenicol resistant; grow with chloramphenicol to retain plasmid
∙Chloramphenicol resistant
∙The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymera which reduces and almost eliminates expression from
transformed T7 promoter containing plasmids when not induced.
∙e Moffatt87 for details of pLysS and pLysE plasmids
7.BNN93
F- tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 mcrB e14-(mcrA-)
hsdR(r
K -m
K
+) λ-
∙Some C600 strains are really BNN93 8.BNN97
∙BNN93 (λgt11)
o A λgt11 lysogen producing phage at 42C
9.BW26434, CGSC Strain # 7658
Δ(araD-araB)567, Δ(lacA-lacZ)514(::kan), lacIp-4000(lacI q), λ-, rpoS396(Am)?, rph-1, Δ(rhaD-rhaB)568, hsdR514
∙This information is from a printout nt by the E. coli Genetic Stock Center with the strain.
∙ B.L. Wanner strain
∙rph-1 is a 1bp deletion that results in a frameshift over last 15 codons and has a polar effect on pyrE leading to suboptimal
pyrimidine levels on minimal medium. (Jenn 1993 J Bact. 175:3401) ∙Δ(araD-araB)567 was former
ly called ΔaraBAD AH33 by Datnko and Wanner
∙Am = amber(UAG) mutation
∙Reference: Datnko and Wanner, 2000, PNAS, 97:6640
NOTE:
∙This promoter driving the expression of lacI was quenced in this strain using a primer in mhpR (upstream of lacI) and a primer in the opposite orientation in lacI. The lac promoter was found to be identical to wildtype. Thus, the -35 quence was GCGCAA not GTGCAA as expected with lacI q. Therefore this strain (or at least the
version obtained from the E. coli Genetic Stock Center) does NOT appear to be lacI q. According to Barry Wanner, this is an unexpected result. -Reshma 13:19, 5 May 2005 (EDT)
∙"We have now confirmed that BW25113, BW25141, and BW26434 are all lacI+, and not lacI q. We thank you for alerting us to the error with respect to BW26434. Apparently, the lacI region was restored to wild-type in a predecessor of BW25113." (from Barry Wanner November 18, 2005)
∙The genotype has been corrected at the CGSC
10.C600
F- tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 λ-
∙There are strains circulating with both e14+(mcrA+) and e14-(mcrA-) ∙General purpo host
∙See CGSC#3004
∙References: Appleyard, R.K. (1954) Genetics 39, 440; Hanahan, D.
(1983) J. Mol. Biol. 166, 577.
11.C600 hflA150 (Y1073, BNN102)
F- thi-1 thr-1 leuB6 lacY1 tonA21 glnV44 λ- hflA150(chr::Tn10) ∙host for repressing plaques of λgt10 when establishing cDNA libraries
刘文华书法∙Reference Young R.A. and Davis, R. (1983) Proc. Natl. Acad. Sci.
USA 80, 1194.
∙Tetracycline resistance from the Tn10 inrtion
12.CSH50
F- λ- ara Δ(lac-pro) rpsL thi fimE::IS1
∙See CGSC#8085
杨幂壁纸∙References: Miller, J.H. 1972. Expts.in Molec.Genetics, CSH 0:14-0;
Blomfeld et al., J.Bact. 173: 5298-5307, 1991.
13.D1210
HB101 lacI q lacY+
14.DB3.1
F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(r
B -, m
高超是什么意思B
-
) ara14 galK2
lacY1 proA2 rpsL20(Sm r) xyl5 Δleu mtl1
宝宝辅食添加∙uful for propagating plasmids containing the ccdB operon.
∙gyrA462 enables ccdB containing plasmid propagation
∙streptomycin resistant
∙appears to NOT contain lacI (bad on a colony PCR) --Austin Che 16:16, 18 June 2007 (EDT)
<biblio>
1.Bernard-JMolBiol-1992 pmid=1324324
2.Miki-JMolBiol-1992 pmid=1316444
</biblio>
15.DH1
endA1 recA1 gyrA96 thi-1 glnV44 relA1 hsdR17(r
K - m
K
+) λ-
∙parent of DH5α
∙An Hoffman-Berling 1100 strain derivative (Melson68)
∙more efficient at transforming large (40-60Kb) plasmids
∙nalidixic acid resistant
运城博物馆∙Reference: Melson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.
16.DH5α
F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80d lacZΔM15
Δ(lacZYA-argF)U169, hsdR17(r K- m K+), λ–
∙An Hoffman-Berling 1100 strain derivative (Melson68)
∙Promega also lists phoA
群龙取水游戏规则
∙nalidixic acid resistant
∙References:
o FOCUS (1986) 8:2, 9.
o Hanahan, D. (1985) in DNA Cloning: A Practical Approach (Glover, D.M., ed.), Vol. 1, p. 109, IRL Press, McLean,
Virginia.
油菜花的作文o Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.
o Melson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.
17.DH10B (Invitrogen)
F- endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ-
∙suitable for cloning methylated cytosine or adenine containing DNA ∙an MC1061 derivative (Casadaban80). Prepare cells for chemical transformation with CCMB80 buffer
∙blue/white lection
∙While DH10B has been classically reported to be galU galK, the preliminary genome quence for DH10B indicates that DH10B (and by their lineage also TOP10 and any other MC1061 derivatives) is
actually galE galK galU+. Dcekiert 16:37, 23 January 2008 (CST)
