专利名称:Method of screening for cell colonies that express a soluble variant of a target protein 发明人:Pär Nordlund,Tobias Cornvik,Sue-Li
Dahlroth,Ro-Marie Knaust
雪中漫步申请号:US10562734
申请日:20040527
公开号:US07718381B2
公开日:
江苏广电集团
太极拳推手
手气脚气20100518
方法的英文专利内容由知识产权出版社提供
早恋检讨书摘要:The prent invention concerns a method of identifying a cell colony which express a soluble variant of a target protein which method compris (a) subjecting the cell colony to conditions which are capable of causing lysis thereof; (b) filtering the lysate through a filter having pores which allow only soluble proteins to pass through the filter; and (c) detecting target protein which has pasd through the filter where the target is not detected an the basis of its own enzymatic activity. The invention further covers the identification of a cell colony expressing a soluble variant of a membrane protein using steps (a) and (b) of the method above. A kit for u in the methods is also covered by the invention.
申请人:Pär Nordlund,Tobias Cornvik,Sue-Li Dahlroth,Ro-Marie Knaust
投资顾问地址:Roslagsgatan 47 Stockholm S-113 54 SE,Fyrskeppsvägen 118 2 Tr Johaneshov S-121 54 SE,Johaneshov SE,Gauting DE
国籍:SE,SE,SE,DE
代理机构:Rothwell, Figg, Ernst & Manbeck
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