Isolation and identification of antioxidant peptides derived from whey protein enzymatic hydrolysate by concutive chromatography and Q-TOF MS 期刊名称： Journal of Dairy Rearch
作者： Qiu-Xiang Zhang,Hui Wu,Yu-Fang Ling,Rong-Rong Lu
作者机构： State Key Laboratory of Food Science and Technology
反劫机
二胡伴奏曲库年份： 2013年
企鹅的英语
期号： 第3期
销售秘书>蜂鸟行动关键词： Whey protein; lipid peroxidation; radical scavenging activity;
antioxidative peptide; Q-TOF MS
摘要：To isolate and identify antioxidant peptides from enzymatically hydrolyd whey protein, whey protein isolate was hydrolyd by different protea (trypsin, pepsin, alcala 2·4L, promatex, flavourzyme, protea N). The hydrolysate generated by alcala 2·4L had the highest antioxidant acti
vities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, superoxide radicals and in a linoleic acid peroxidation system induced by Fe2+. The IC50values of DPPH and superoxide radical scavenging activities of the hydrolysate decread significantly (6·89 and 38·88%, respectively) after treatment with macroporous adsorption resin. Seven different peptides showing strong antioxidant activities were isolated from the hydrolysate using concutive chromatographic methods including gel filtration chromatography and high-performance 读书小报五年级
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