Laboratory Manual for Physiological Studies of Rice SHOUlCHl YOSHIDA DOUGLAS A. FORNO JAMES H. COCK KWANCHAI A. GOMEZ
THIRD EDITION
THE INTERNATIONAL RICE RESEARCH INSTITUTE 1976
LOS BAÑOS, LAGUNA, PHILIPPINES MAIL ADDRESS P.O. BOX 933 MANILA, PHILIPPINESqq悄悄话在哪里
FOREWORD
This manual is primarily intended for students of crop physiobgy and agronomy.
The procedures given in the text of this manual are particularly suited for routine chemical analysis and physiological studies of the rice plant. Considerable attention is given to technical matters involved in the procedures.
中国幼教The equipment ction of each chapter lists only special items needed for the procedures discusd. Ordinary laboratory equipment is not listed.
Shouichi Yoshida Douglas A. Forno James H. Cock Kwanchai A. Gomez
CONTENTS
Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6
Chapter 7 Chapter 8 Chapter 9 Chapter 10 Chapter 11 Chapter 12 Chapter 13 Chapter 14 Chapter 15 Chapter 16 Chapter 17 Chapter 18 Sampling and sample preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 General directions for chemical analysis of rice
tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 Analysis for total nitrogen (organic nitrogen) in网络祭祖
plant tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Procedures for routine analysis of phosphorus,洗衣机排名
iron, mangane, aluminum, and crude silica in
plant tissue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 An EDTA method for routine determination of
calcium and magnesium in plant tissue and soil
solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Procedures for routine analysis of zinc, copper,
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mangane, calcium, magnesium, potassium, and
京酱肉丝做法
sodium by atomic absorption spectrophotometry
学生作业评语and flame photometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Dithizone test for heavy metals in solution . . . . . . . . . . . . . . . . . . . . . 35 Analysis of boron in plant tissue and water . . . . . . . . . . . . . . . . . . . . . 38 Determination of chlorine in plant tissue . . . . . . . . . . . . . . . . . . . . . . 41 Determination of chlorophyll in plant tissue . . . . . . . . . . . . . . . . . . . . 43 Determination of sugar and starch in plant tissue . . . . . . . . . . . . . . 46 Determination of total 14 C in plant tissue . . . . . . . . . . . . . . . . . . . . . 50 Determination of 14 C-labelled sugar in plant tissue . . . . . . . . . . . . . 53 Determination of 14 C-labelled starch in plant tissue . . . . . . . . . . . . 56 Assimilation of 14 CO 2 by intact plants in the field . . . . . . . . . . . . . . 58 The safranin-phenol method for detection of silici-
fied cells in rice tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 Routine procedures for growing rice plants in cul-
ture solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Measurement of light intensity and light trans-
mission ratio . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Chapter 19 Chapter 20 Chapter 21 Chapter 22 Chapter 23 Appendix 1 Appendix 2 Appendix 3 Appendix 4 Measurement of leaf area. leaf area index. and
leaf thickness . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Measurement of leaf angle (leaf openness) . . . . . . . . . . . . . . . . . . . . . Measurement of grain yields . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Measurement of yield components . . . . . . . . . . . . . . . . . . . . . . . . . . . . Identification of unfertilized grains . . . . . . . . . . . . . . . . . . . . . . . . . . . Abbreviations ud in this manual and their
送客松图片meanings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
A list of chemical suppliers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
A list of equipment suppliers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
A list of isotope suppliers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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