pricedExpression patterns of ubiquitin, heat shock protein 70, α‐actin and β‐actin over the molt cycle in the abdominal muscle of marine shrimp Litopenaeus
姜育恒再回首vannamei
马云年会
个人形象管理期刊名称: Molecular Reproduction and Development
作者: Jo Renato O Cesar,Jinzeng Yang
年份: 2010年
期号: 第5期
关键词: shrimp;L. vannamei;muscle;mRNA expression;actin;ubiquitin;Hsp70
摘要:Crustacean muscle growth is discontinuous due to molt cycle. To characterize molt-related gene expression patterns, we studied the mRNA levels of molecular chaperone-ubiquitin and heat shock protein 70 (Hsp 70) in comparison网课迟到检讨
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with muscle protein -actin and -actin in marine shrimp Litopenaeus vannamei . Total RNA from abdominal muscle was isolated from 3-month-old animals in six different molt stages. The mRNA levels of target genes were detected by rever-transcripta-multiplex PCR and expresd as the ratio to elongation factor-1. Ubiquitin mRNA levels were relatively steady over all stages of the molt cycle.
Hsp70 levels were not detectable in early postmolt and late premolt stages, but showed a progressive increa from late postmolt to intermolt stages. Expression levels of -actin gene were lower during postmolt, reached
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