pricedExpression patterns of ubiquitin, heat shock protein 70, α‐actin and β‐actin over the molt cycle in the abdominal muscle of marine shrimp Litopenaeus
姜育恒再回首vannamei
马云年会
个人形象管理期刊名称： Molecular Reproduction and Development
作者： Jo Renato O Cesar,Jinzeng Yang
年份： 2010年
期号： 第5期
关键词： shrimp;L. vannamei;muscle;mRNA expression;actin;ubiquitin;Hsp70
摘要：Crustacean muscle growth is discontinuous due to molt cycle. To characterize molt-related gene expression patterns, we studied the mRNA levels of molecular chaperone-ubiquitin and heat shock protein 70 (Hsp 70) in comparison网课迟到检讨
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with muscle protein -actin and -actin in marine shrimp Litopenaeus vannamei . Total RNA from abdominal muscle was isolated from 3-month-old animals in six different molt stages. The mRNA levels of target genes were detected by rever-transcripta-multiplex PCR and expresd as the ratio to elongation factor-1. Ubiquitin mRNA levels were relatively steady over all stages of the molt cycle.
Hsp70 levels were not detectable in early postmolt and late premolt stages, but showed a progressive increa from late postmolt to intermolt stages. Expression levels of -actin gene were lower during postmolt, reached
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