TBI后脑细胞内Ca2水平动态变化与CGRP的相关性研究
山西医科大学硕士学位论文
TB I后脑细胞内Ca2+水平动态变化与CGRP的相关性研究
摘要
目的 探讨钙离子在脑损伤后引起的一系列病理生理改变，进一步探讨钙离子与脑损 伤程度之间的关系及创伤性颅脑损伤(TBI)后钙离子动态变化与降钙素基因相关肽 (CGI心)水平之间的相关性。
方j法 通过对Wistar大鼠硬膜外自由落体打击法来制备颅脑损伤动物模型，采用激光 扫描共聚焦显微镜和酶联免疫吸附实验(ELISA)分别对对照组(36只)和实验组(108 只)测定不同时间点脑细胞内钙离子浓度和血浆中CGRP水平，统计方法采用单因素方差 分析、两因素析因设计方差分析、Pearson简相关分析。
结果对照组大鼠脑细胞内钙离子荧光强度维持在较低的水平，实验组各组大鼠不同 时间点脑细胞内钙离子荧光强度与对照组相比，均有显著性差异(P<0．01)，在伤后2小时 左右即有明显升高，约在24小时达到高峰，48小时左右趋于下降。在不同程度脑损伤组 间，重度组脑细胞内钙离子荧光强度水平较轻中度组显著升高口<O．05)，随着损伤程度的
加重，大鼠脑细胞内的钙离子荧光强度水平逐渐增高。实验组各组大鼠不同时间点血浆中
降钙素基因相关肽水平与对照组相比，均有显著性差异(P<0．05)，实验组内大鼠脑损伤后 血浆中的CGRP水平总体先升高后降低，再缓慢升高。在不同程度脑损伤组间，重度组 CGRP水平较轻中度组显著降低(P<O．05)，随着脑损伤程度的加重，大鼠血浆中降钙素基因 相关肽(CGRP)水平逐渐降低。
结论证实脑损伤后脑细胞内存在着钙超载，钙离子在脑损伤后继发性损害中起着重
要的作用；TBI后脑细胞内钙离子水平的动态变化与CGRP之间存在负相关性。
关键词：钙超载降钙素基因相关肽创伤性颅脑损伤共聚焦显微镜
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山西医科大学硕士学位论文
The dynamic changes of brain intracellular Ca2+levels and the relationship of the Ca+and CGRP after TBI
Abstract
Ob 吊死在一棵树上j ect i ve The calcium was investigated in下载设置 the traumatic brain injury(TBI)caud by a
ries of path physiological changes，and further the relationship between the the calcium ions
and the calcitonin gene-related Peptide(CGRP)was rearched．
Methods Animal model oftraumatic brain injury were constructed by epidural freefall using Wistar rats．The calcium concentration and the plasma CGRP levels were got by lar scanning cortfocal microscope and enzyme-linked immunosorbent assay(ELISA)，respectively on the
normal control group(36 not against)and experimental group(108 against)at different time points，such as 2h,6h,12h，24h,4Sh,72h．The data was analyzed by single-factor analysis of variance；two factors factorial design analysis ofvariance，Pearson correlation analysis
Resu I ts In the control group calcium fluorescence intensity remained at a relatively low level in the brain cell，there were significant differences of the brain cells calcium fluorescence intensity between the experimental group and the魔兽世界3冰封王座 control group口<O．0 1)，that is significantly higher about two hours aRer mjury，peal【at about 24 hours and a downward trend about 48 hours．
There were significant differences of the brain眼睛是心灵的窗户 cells calcium fluorescence intensity between the
vere group and the mild and moderate group口<0．05)．The rat brain cells calcium fluorescence intensity一个骨一个果 level gradually incread with the degree of injury．There were significant differences of the plasma CGRP levels between each experimental group and the control group at different time points口<O．05)．In the experimental groups the level of CGRP incread at first and then decread and incread slowly．There were significant differences of the plasma CGRP levels between∞vere group and the mild-moderate group口<O．05)．The plasma CGRP levels decread
、^，inl the degree of brain damage incread．The 自拍文案brain intracellular calcium ion concentration and
the CGRP level were negatively correlated after brain injury．
Cono I US i on Calcium overload played an important in the brain condary damage after TBI．
There was a negative correlation between CGRP and brain intracellular calcium level．CGRP could reduce the calcium concentration in traumatic brain injury．
Key words：calcium overload，calcitonin gene-related pcptide，traumatic brain injury，confocal microscope
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山西医科大学硕士学位论文
英文缩略词表
(Abbreviation)
ASCF Artificial cerebrospinal 人工脑脊液
BBB Blood brain barrier 血脑屏障
CBF Cerebral blood flow 脑血流
CGRP Calcitonin gene related peptide 降钙素基因相关肽 CaM Calmodulin 钙调蛋白
DAI Diffu axonal injury 脑弥漫性轴索损伤
DRG Dorai root ganglia 背根神经节
DMSo Dimethyl sulfoxide 法律论文无水二甲基亚砜
ET Endothelin 内皮素
lCP Intra cranial pressure 颅内压 LSCM Lar scanning confocal microscope 激光共聚焦显微镜 NGF Nerve growth factor 神经生长因子 RoCC Recepter-operated calcium channel 受体操纵性通道
SAH Subarachnoid 人民的名义3蛛网膜下腔出血
TBI Traumatic brain injury 创伤性脑损伤
