函调证明
Multiplex polymera chain reaction with DNA pooling: a cost-effective strategy of genotyping rare
blood types
期刊名称： Transfusion Medicine
文献述评王羲之的故事墨池作者： He, Y.-L.,Gao, H.-H.,Ye, L.-Y.,Guo, Z.-H.,Wang, P.,Zhu, Z.-Y.
大学生的自我介绍年份： 2013年
期号： 第1期
关键词： blood typing; DNA pool; PCR‐SSP
楚门的世界情书文案摘要：This work aims to develop a multiplex polymera chain reaction combined with DNA pooling for mass screening for rare blood王闿运
types.BackgroundThe differences in most blood group antigens are associated with single-nucleotide polymorphisms (SNPs), which are ud in detecting blood antigen expression at the molecular level. H
owever, all existing quence-specific primers polymera chain reaction (PCR-SSP) assays for blood typing genotype one or
威比特veral SNPs individually. DNA pooling is a way that reduces the amount of
genotyping required.MethodsA nsitive multiplex PCR-SSP assay testing pooled DNA was established to detect the rare Fyb and S alleles. It was applied to screen a total of 4490 donor samples via testing 898 DNA pools. The samples in the positive pools were further tested individually. Then the positive samples,
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