Expression of ZO-1 and occludin at mRNA and protein level during preimplantation development of the pig parthenogenetic diploids 期刊名称： Zygote
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dominate翻译作者： Xu, Shangdan,Lee, Jibak,Miyake, Masashi
英语专业毕业论文年份： 2012年
期号： 第02期
关键词： Parthenogenetic embryo; Pig; Preimplantation; Tight junctionveral是什么意思
摘要：Expression of mRNAs and proteins of ZO-1 and occludin was analyzed in pig oocytes and parthenogenetic diploid embryos during preimplantation development using real-time RT-PCR, western blotting and immunocytochemistry. All germinal vesicle (GV) and metapha (M)II oocytes and preimplantation embryos expresd mRNAs and proteins of ZO-1 and occludin. mRNA levels of
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少儿辅导both ZO-1 and occludin decread significantly from GV to MII, but incread at the 2-cell stage followed by temporal decrea during the early and late 4-cell stages. Then, both mRNAs incread afteasterday
er compaction. Relative concentration of
zo1u03b1u2212 was highest in 2-cell embryos, while zo1u03b1+ was expresd
from the morula stage. Occludin expression greatly incread after the morula stage and was highest in expanded blastocysts. Western blotting analysis 感叹句练习
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