STOCK SOLUTIONS
Prepared by Mrs Andie Hall
3M NaOAc pH 5.2
49.22g molecular biology grade NaOAc (mw 82.03)
Dissolve in 100ml analar water.
Adjust pH to 5.2 (or 4.6 for beetle group) with glacial acetic acid and adjust volume to 200ml with analar water.
Autoclave and store at 4°C.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
10x Tris-borate (TBE)
Tris 43.2g
Orthoboric acid 22g
EDTA 0.5M pH8.0 16ml
Made up to 400mls with distilled water
Autoclave .
Wear lab coat, latex gloves, dust respirator and safety spectacles.
50x TAE Tris ba 242g GAA 57.1ml EDTA 0.5M pH 8.0 100ml Made up to 1L with water 10x Tris-acetate (TAE) Tris ba 19.4g Glacial acetic acid 4.6ml EDTA 0.5M pH8.0 16ml Made up to 400mls with distilled water
Autoclave.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
6x Orange G loading buffer
0.25% Orange G in 30% Glycerol
(30ml glycerol + 70ml water + 0.25g Orange G)
Wear lab coat, latex gloves, dust respirator and safety spectacles.
6x Gel loading buffer
0.025g Bromophenol blue
0.025g Xylene cyanol FF
3ml Glycerol
Water to 10ml
Wear lab coat, latex gloves, dust respirator and safety spectacles.
GelRed loading buffer
417ul 6x Gel loading buffer (either colour)
82ul ddH20
1ul GelRed
U 1ul of buffer to 3ul of PCR product.
PCR Buffer 10x
8.116g Tris-HCl 670mM
0.61g MgCl2 30mM
2.2g (NH 4)SO 4 166mM
Make up to 100ml with water
Adjust to pH8.3
Wear lab coat, latex gloves, dust respirator and safety spectacles.
Binding Buffer (to clean PCR products from Millipore plates)
33.4g Guanidine HCl (Qbio GUAC0100)
22.5ml/1.76g 400mM MES (free acid)
2.5ml/0.22g 400mM MES (Na salt)
Make up to 50ml with water.
Adjust to pH6.4-6.8 (<7) with NaOH (solid).
Wear lab coat, latex gloves, dust respirator and safety spectacles.
2.5N Hydrochloric acid
215ml 1.18SG HCl made upto 1000ml with distilled water.
我们敢 we dareWear lab coat, latex gloves, dust respirator and safety spectacles.
0.5M Sodium Hydroxide
8g NaOH (mw 40.0)
Add to 300ml distilled water, when dissolved adjust the volume to 400ml with distilled water. Wear lab coat, latex gloves, dust respirator and safety spectacles.
bind
2M Tris-HCL pH 7.5
96.91g Tris HCl (mw 121.1)
to 250ml distilled water.
Adjust pH to 7.5.
Make up to 400ml with distilled water.
Autoclave and store at 4°C.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
10M Sodium Hydroxide
80g Sodium Hydroxide (mw 40.0)
made up to 200ml with Distilled water.
(Or 200g into 500ml)
Wear lab coat, latex gloves, dust respirator and safety spectacles.degen
10% w/v SDS (sodium dodecyl sulphate)
40g SDS (mw 288.4)
Dissolve in 400ml distilled water.
Do not autoclave as polymers form.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
20 X SSC
70.12g NaCl (mw 58.44)
35.28g Sodium citrate (mw 294.1)
Add to 300ml distilled water, when dissolved adjust volume to 400ml with distilled water. May need to adjust to pH 7.2. Autoclave and store at room temperature.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
10x Grinding buffer
0.1M Tris-HCl pH7.5
0.6M NaCl
0.1M EDTA
Autoclave, store at 4o C
Wear lab coat, latex gloves, dust respirator and safety spectacles.
季风图
2x SDS buffer
0.8M Tris-HCl pH 9.0
0.27M EDTA
Autoclave, store at 4o C.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
20x Spermine/Spermidine
3mM Spermine
3mM Spermidine
(Make up in autoclaved dH20 and store 1ml aliquots in Eppendorf tubes at -20O C).
Wear lab coat, latex gloves, dust respirator and safety spectacles.
10x TE buffer
100mM Tris-HCl pH8.0
10mM EDTA (pH 8.0, if from stock solution)
(1.211gTRIS + 0.372g EDTA into 100ml)
Wear lab coat, latex gloves, dust respirator and safety spectacles.
0.5M EDTA pH 8.0
74.44g EDTA (mw 372.24)
To 250ml distilled H20.
Adjust pH to 8.0 and the EDTA will start to dissolve once dissolved adjust volume to
400ml.
Autoclave and store at 4o C.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
100X Denhardt's solution
Ficoll 4g (2%)3rd
Polyvinyl pyrrolidone 4g (2%) 2nd
Bovine Serum Albumen 4g (2%) 1st
Add to 150ml dH20, when dissolved adjust volume to 200ml with dH20.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
2M Tris Ba
96.91g Tris ba (mw 121.1)
Add to 300ml dH20.
周一到周日的英文Adjust volume to 400ml with dH20.
Autoclave and store at 4°C.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
Proteina K
1mg/ml in 0.1M Tris HCl pH 7.5
OR 0.05g Proteina K + 0.25ml 2M TRIS HCL + 4.75ml Water.
写作文的顺序Aliquot into 1ml lots and store at -20°C
Wear lab coat, latex gloves, dust respirator and safety spectacles.
X-Gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactosida)
20mg/ml of X-Gal in dimethylformamide. (0.2g into 10ml)
Wrap the tube in foil to prevent damage by light and store at 20°C.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
IPTG (Isopropylthio-ß-D-galactosida) 0.1m
0.6g IPTG dissolved in 20ml dH20. Adjust volume to 25ml with dH20 and sterili by filtration through a 0.22 micron disposable syringe filter. Dispen solution into 1ml aliquots and store at 20°C.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
8M Potassium acetate
157.02g KOAc (mw 98.14)
in 200ml of distilled water.
May need to adjust to pH 7.5, using GAA.
Autoclave and store at 4o C.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
北京计算机培训学校PBS 10x
40g NaCl + 1g KH2PO4 + 6g Na2HPO4 (anhydrous) + 1g KCl
Make up to 500ml with water.
LB agar (for plates)
rzm10g Tryptone
5g Yeast extract
cry是什么意思10g NaCl
into 1litre.
Agar – 3g/200ml; 7.5g/500ml
Autoclave
LB agar (for screening plates)
Make LB agar as above, but once hand-hot, add 5ml of a 0.1m IPTG solution (Filtered) and 1.6ml of a 50mg/ml X-Gal solution.
LB Medium (liquid)
10g Tryptone
5g yeast extract
5g NaCl
mitreMake up to 1litre with water. Adjust to pH7.0 with NaOH. Autoclave.
SOC medium
2g Tryptone
0.5g yeast extract
1ml NaCl 1M
0.25ml KCl 1M
Autoclave.
When cool add:
1ml Mg2+ solution (2.033g MgCl2-6H20 + 2.465g MgSO4-7H2O in 10ml H2O) filter sterilid 1ml 2M gluco solution filter sterilid.
10M Ammonium Acetate (CH3COONH)
192.70g Ammonium acetate into 250ml water.
Heat slightly to dissolve, filter, sterilize.
PEG/NaoAc 26.6%
13.3g PEG 8000
333mcl MgCl2 1M
10ml NaoAc pH4.8, 3M
Make up to 50ml with water.
Wear lab coat, latex gloves, dust respirator and safety spectacles.
NZCYM Media
10g
5g NaCl
5g Yeast extract
1g Casamino acids
2g MgSO4.7H2O
Make up to 1 litre, pH7.0, Autoclave.
Qiagen buffer P1
50 mM Tris·Cl, pH 8.0
10 mM EDTA
100 µg/ml RNa A
After RNa A addition, the buffer should be stored at 2–8°C.
Qiagen Buffer P2
200 mM NaOH
1% SDS (w/v)
Store at room temperature. Qiagen Buffer P3
3.0 M potassium acetate, pH 5.5 Store at room temperature.
