培养基及成分
1、Acetobacter Medium (醋酸菌培养基)
Gluco (葡萄糖) 100g Yeasst extract (酵母膏) 10g
CaCO3 20g Agar (琼脂) 15g
新东方教师戚颖艳照Distilled water (蒸馏水) 1000ml Adjust (调) pH to 6.8
适用范围:恶臭醋酸杆菌混浊变种
箱子的英文2、 Nutrient Agar (营养肉汁琼脂)
singlePepton (蛋白胨) 5g Beef extract (牛肉膏) 30g
NaCl 5g Agar (琼脂) 15g
Distilled water (蒸馏水) Adjust (调) pH to 7.0-7.2
[Note]:When cultivation of Bacillus,5mg of to MnSO4.H2O may be added . shameonyou
It is favorable to promote spore formation .
适用范围:产气气杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡状芽孢杆菌蕈状变种、地衣形芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌、尘埃芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、
枯草芽孢杆菌、枯草芽孢杆菌深黑变种、苏云金芽孢杆菌、苏云金芽孢杆菌蜡螟亚种(青虫菌)、苏云金芽孢杆菌戈尔斯德变种、苏云金芽孢杆菌猝倒亚种、产氨短杆菌、黄色短杆菌、谷氨酸棒状杆菌、北京棒杆菌、大肠埃希氏菌(大肠杆菌)、铜绿假单胞菌(绿脓杆菌)、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、藤黄八叠球菌、亚黄八叠球菌、尿素八叠球菌、金黄色葡萄球菌、运动发酵单孢菌
3、Azotobacter Medium (固氮菌培养基)
KH2PO4 0.2g K2HPO4 0.8g
MgSO4.7H2O 0.2g CaSO4.2H2O 0.1g
Na2MoO4.2H2O Trace(微量) Yeast axtract(酵母膏) 0.5g
Mannitol(甘露醇) 20g FeCl3 Tract(微量)
Distilled water (蒸馏水) 1000ml Agar (琼脂) 15g
Adjust (调) pH to 7.2
适用范围:固氮菌、胶质芽孢杆菌
4、Corn Meal Medium (玉米粉培养基)
Maize flour (玉米粉) 5g Peptone (蛋白胨) 0.1g
Gluco (葡萄糖) 1g Tap water (自来水) 1000ml
[Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮金融英语证书考试1小时,分装入18ⅹ18毫米试管,每管深度达6厘米。15磅再次灭菌15小时。)
5、Lactic-bacteria Medium I (乳酸菌培养基 I )
Yeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5g
Gluco (葡萄糖) 10g KH2PO4 2g
Tomato juice (西红柿汁) 100ml Tween (吐温) 80 0.5ml
Distilled water (蒸馏水) 900ml pH 7.0
适用范围:植物乳杆菌(胚芽乳杆菌)、嗜热乳酸链球菌
6、Lactic-bacteria Midium Ⅱ (乳酸菌培养基 Ⅱ)
Lacto-cain peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10g
Yeast extract (酵母膏) 5g Gluco (葡萄糖) 5g
Tween (吐温) 80 1g K2HPO4 2g
Na-acetate (醋酸钠) 5g Diamine citrate (柠檬酸二胺) 2g
MgSO4.7H2O 0.2g MnSO4.H2O 0.05g
Distilled water (蒸馏水) 1000m pH 6.5-6.8
适用范围:植物乳杆菌(胚芽乳杆菌)
新年快乐的英文怎么写
7、Peotone Gluco Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培养基)
Peptone(蛋白胨) 10g Yeast extract (酵母膏) 5g
Gluco (葡萄糖) 1g Distilled water (蒸馏水) 1L
8、Glycerol Agar (甘油琼脂)
Peptone (蛋白胨) 5g Beef extract (酵母膏) 3g
Glycerol (甘油) 20g Top water (自来水) 1000ml
Agar (琼脂)murder 15g pH 7.0-7.2
9、Rhizobium medium (根瘤菌培养基)AS 9
Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml
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Mannitol (甘露醇) 10g Agar (琼脂) 15g
Distilled water (蒸馏水) 800ml pH 7.2
[Note]快速美白牙齿的方法:Soil extract:Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的制法:取土壤50克,加水200毫升,15磅蒸煮1小时,经滤纸过滤后加水补足到200唐老鸭编年史毫升。)
适用范围:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、
大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌