Early Molecular Diagnosis of Acute Chagas Dia After Transplantation With Organs Fromsurvival
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bmTrypanosoma cruzi–Infected Donors经典史诗电影
期刊名称： American Journal of Transplantation
作者： C I Cura,R Lattes,C Nagel,M J Gimenez,A G Schijman
年份： 2013年
期号： 第12期
关键词： DISCRETE TYPING UNITS;LIVER-TRANSPLANTATION;HEART-
TRANSPLANTATION;BLOOD-
SAMPLES;PCR;TRANSMISSION;REACTIVATION;POPULATIONS;LINEAGES;IDENTIFICATION
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摘要：Organ transplantation (TX) is a novel transmission modality of Chagas dia. The results of m
olecular diagnosis and characterization of Trypanosoma cruzi acute infection in nave TX recipients transplanted with organs from infected decead donors
lucky是什么意思are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and quentially studied for detection
奥运新闻of T. cruzi by means of kinetoplastid DNA polymera chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight
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