美国教师节
山西医科大学学报，2020年88月，第58卷第88期-1102-四级考试准考证打印入口官网
荧光增白剂CXT和CBS的物理化学性质及细胞毒性
姚苏霞8，薛红波"，王真祥5，杜江锋1(山西医科大学医学影像学院，太原03/008；6山西省疾病预防控制中心卫生检验二科;2山西青山化工有限公司研发部；*通信作者,)大贺典雄
摘要：目的研究荧光增白剂CXT和CBS的物理化学性质及其对人支气管上皮细胞16HBE和人皮肤角质细胞Hid的生物安全性。方法利用高效液相色谱仪检测紫外吸光度反映荧光增白剂的纯度，利用动态光散射仪检测荧光增白剂分子的尺寸和电荷，使用分光测色仪检测荧光增白剂对棉布的增白效果。以人支气管上皮细胞16HBE和人皮肤角质细胞Hid 为研究对象，利用酶标仪和MTT试剂检测细胞液在490nm处的吸光度反映细胞活力，利用显微镜观察细胞膜表面形态变化，利用流式细胞仪检测细胞液荧光强度反映细胞凋亡情况。通过设立空白对照组,50,100,200m g/mi荧光增白剂CXT和CBS 组，研究荧光增白剂CBS和CXT对这两种细胞的毒理学效应。结果荧光增白剂CXT和CBS主要成分的纯度在97%左右，对棉布均有良好的增白强度，分别达到97.67%和97.67%，其表面具有负电荷和纳米级的分子尺寸。研究结果显示，与空白对照组的细胞相比，荧光增白剂CBS在60m g/mi时16HBE和Hid细胞活力分别降低到80.0%和90.1%，荧光增白剂CXT在100m g/mi时10HBE和Hdd细胞活力分别降低到88.5%和93.2%，但这两种细胞形态均未发生明显变化。细胞凋亡的流式分析结果也证实，即使在200m g/mi的荧光增白剂溶液作
tell me a lie>eqs用下，细胞也未发生明显的凋亡。结论荧光增白剂CXT和CBS表面的负电荷使它们具有良好的水溶性，纳米级的分子尺寸使其染色效果更为持久。在日常使用情况下(洗涤剂中的荧光增白剂的添加量为4-40m/mU，荧光增白剂中无论是CBS还是CXT均不会对呼吸道或者皮肤表面的细胞造成毒性损伤，具有良好的生物安全性。
关键词：荧光增白剂CXT；荧光增白剂CBS；细胞活力；细胞凋亡；毒理学
genetically中图分类号：R994文献标志码：A文章编号：1027-6616(2222)16-1105-08DOI：12.15755/j.imn.1027-6616.2222.16.210
发型设计学校误会的英文Toxicity of auorescencenS brightenee CXT and CBS witO vv V ous physiochemical propertiespitbull新歌
YAO Suxia1,XUE Hongbo6*,WANG Zhexxiang2,DU Jiaugfeeg1(1Collepe rf Medical Omaging,Shanxi Medical University,Taiyuak 030001,Chaia；Senn Department cf Sanitarg Inspection,Shanxi Provincial Center for Didss Controi oo C Prevention ；3Shanxi Qing-sPan ChemicaI Industrg Co.La；*Corfspondinx antaor,E-'mail■)
w hotelAbstract:Objectiee To stuPy the physicochemical properUes of Cuomsceet whiteeing ageets CXT and CBS and their biolovicai safety to humac bronchial eyithelial cells10HBE and humac s Pix Uemti
nocytes HaCaT.Hph-peUormauce lipuid chmmatoamphy was ud to Uetermiue the purity of the Cuomsceet whiteeing ageet by meass/ng UV absordauce,and a Uypamic light scatte/ng was ud to measure the size and charye of the Cuomsceet whitening ageet,and the whiteeing effect of Cuomsceet whiteeing agent on cotton fab/c was tested by spectmphotomeWu.Humac bronchial eyitheVai cell10HBE and humac OemtiuocyW HaCaT were ud as the rearch objects.The absorption value at490nm was UeWcWd by enzyme labeled insWumeet and MTT reageet to evaluate the cell viabi/ty,the morphoUgical chauges of cell membrace were obrved by microscope ,and the apopWsis of—Ils was UeWcted by Cow cytometp.The cells were divided into blaud control group,54mf/ml,140mf/ml,200ml/ml Cuomsceet whiteeing ageet CXT and CBS gmups.The toxicoUgicai effects of Cuomsceet whiteeing ageets CBS and CXT on10HBE and HaCaT cells were obmed. Resulta The pu/ty of the maix componeets of the Cuomsceet b/ghweers CXT and CBS was about97%.The whitening sWength for cotton cloth was99.96%and99.69%,mspectivelu The Cuomsceet whiteeing ageets CXT and CBS hab uepative charye on the ssUace and uauo-level of molechlas size.Compared to bUcd control gmup,the viabili/es of10HBE and HaCaT cells were decread to 80.0%dd90.1%ix140mt/ml CBS gmup,and the viabili/es of16HBE dd HaCaT cells were decread W88.5%and93.7%ix 140mt/ml CXT gmup,mspectiveU,but the morphology of the two Uinds of cells showed uo significaut change.The Cow cytometric analysis m s uits aUe confirmed tha
t the cells did uot uxderyo obvious apoptosis even uxdes200ml m l fluomsceet whitening ageet soU-tion.Conclusion The uepative charye on the ssUace mabes CXT and CBS have goob water soUPi/ty.The uauo-level of molechlas
作者简介：姚苏霞，女,1086-07生，硕士，讲师,E-mail：
收稿日期：2020-09-22
