big wolfFluorescence & Luminescence What is the difference…chloe vigny
-Enzyme
-Triggeringknow是什么意思
reagent
Light
忖度Fluorescence
Different type of applicationsdoomed
«Standard» fluorescence Ex. DNA quantitation
FRET (fluorescence resonance energy transfer) Accessibility of molecules, distances (= quenching) «Flash» Fluorescence Ex. Calcium uptake Follow and quantitate fast events
gosh什么意思TRF (time resolved fluorescence)
Background free
TR-FRET (TRET)
traffic lightsfatherCombination of FRET and TRF
FP (fluorescence polarization)
Mobility of molecules
>> To Luminescence
Fluorescence
«Standard » fluorescent applications
Enzyme Antibody •
Direct quantitation (NADH, NADPH)•
GFP –Molecular Biology •
Fluorescent substrates (enzyme activity monitoring, Fluorescent ELISA …)•
大连会计培训六级分数的分布情况Immuno-fluorescence.•
Labeled antibodies ud to locate or quantitate antigens in complex molecular environments (cells…)•Labeling (DNA, protein quantitation)…
Fluorescence
FRET
Fluorescence Resonance Energy Transfer
