专利名称：In-situ hybridization to detect nucleic acid quences in morphologically intact cells 发明人：Robert H. Singer,Jeanne B. Lawrencebolle
申请号：US07/257198
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申请日：19881013
公开号：US04888278A
公开日：
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摘要：Improved methodologies for in-situ hybridization and detection of hybridized nucleic acid quences in cell cultures and tissue ctions are provided which offer an increa of speed, nsitivity, and simplicity unavailable in previously known techniques. The invention detects specific nucleic acids of interest, particularly RNA quences, within cells and tissues utilizing DNA of a particular size as a probe to find tho quences which are held substantially in common between the cell or tissue and the probe. The cells are fixed preferably in paraformaldehyde and then hybridized using a hybridization fluid for not less than 10 minutes but not substantially more than 24 hours. A variety of identifying labels are attached to the probe which permit quick and rapid detection via measurement of radioactive isotope decay or by colorimetric detection of enzymatic reaction products. The invention is intended for u as a diagnostic kit in
vicpclinical/diagnostic laboratory testing facilities in that it permits a relatively unskilled person to accurately and reproducibly detect a few molecules of a specific nucleic acid of interest in-situ in 10 minutes.
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申请人：UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER
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