developer是什么意思Determination of Gluco oxida Activity
1Principle
Under the effect of gluco oxida,gluco and oxygen respons are generated by gluco acid and hydrogen peroxide.Under the effect of hydrogen peroxide enzyme, hydrogen peroxide and colorless prototype o-Dianisidine respons are generated by water and red oxidation o-Dianisidine.
2Reagents and Solutions
In addition to special instructions,the reagents ud for the analysis of pure,water are in line with the provisions of the three levels of water in GB/T6682.
（1）Phosphate buffer solution,pH6.0
Take16.61g NaH2PO4·2H2Oand35.82g Na2HPO4·12H2O,dissolved in water without CO2,dilution to1000mL.
（2）o-Dianisidine l methanol buffer solution
阿拉伯语字母Take1g o-Dianisidine into100ml methanol solution and agitation dissolution.Now with the current,take0.1ml o-Dianisidine l methanol buffer solution into12ml phosphate buffer solution and mix them uniformity.
（3）Gluco solution,180g/L
Take18g gluco;add water to dissolve and constant volume to100ml.
（4）Horradish Peroxida(HRP)solution,0.0033%
决赛英文Take10mg HRP and add30ml water to dissolve it.
男性英文名字3Apparatus and equipment
（1）Analytical balance:The amount of n is0.0001g
（2）pH meter:accurate to0.01
（3）Magnetic stirrer:heating function
（4）Electromagnetic oscillator
（5）Centrifuge:4000r/min
（6）Thermostatic bath:temperature control range between30℃and60℃,the accuracy is0.1℃.
（7）Stopwatch：The error per hour is no more than5s.
（8）Visible light spectrophotometer:Detection of the absorbance range of350-800nmbitchy
（9）Transferpettor：1μL
4Experimental methods
（1）Preparation of enzyme liquid
Solid products：
美味的英语Dissolution of1g sample with phosphate buffer solution was100mL.Solution was magnetic stirring30min and centrifugal5min with6000r/min.The
supernatant was diluted with phosphate buffer solution.
Liquid product:
1ml sample was diluted with phosphate buffer solution.
Note:the diluted sample measured enzyme solution of△A value in0.15-0.65.（2）Determination procedure
Take two clean tubes,in order to add the following reagents.
Blank Sample
A.2.5ml o-Dianisidine l methanol buffer solution A.2.5ml o-Dianisidine l methanol buffer solution
B.0.3ml Gluco solution B.0.3ml Gluco solutionishow怎么样
C.0.1ml HRP solution C.0.1ml HRP solution
D.Mixing solution,constant temperature water bath at30C5min D.Mixing solution,constant temperature water bath at30C5min
E.0.1ml water,mix E.0.1ml sample,mix
F.460nm,zero F.460nm,Read value A0,3min after
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reading the value A1,△A=A1-A0 Each sample should be taken for analysis of two parallel samples;the difference of absorbance value is not more than0.02as effective parallel value.
（3）Calculation
The enzyme activity of gluco oxida was expresd by X;unit is U/g or U/ml.
A1——Arithmetic mean value of light absorption after reaction of parallel sample A0——Arithmetic mean value of light absorption before reaction of parallel sample
N—Dilution multiple of sample
11.3—Extinction coefficient
t—Sample and substrate reaction time，min事业单位会计制度
0.1—Sample volume，ml
m—Sample weight，g
The results are expresd as integers.
